ATTO 700 Tetrazine
Product key features
- Ex/Em: 699/715 nm
- Extinction coefficient: 120,000 cm-1M-1
- Reactive group: Tetrazine
- Click Chemistry Conjugation: Labels TCO-modified biomolecules under copper-free conditions with fast reaction kinetics
- High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
- Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy
Product description
ATTO 700 is a near-infrared fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 670-715 nm wavelength range. As a zwitterionic compound, ATTO 700 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make ATTO 700 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, ATTO 700 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.
ATTO 700 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.
Spectrum
References
Authors: d'Astous, Élodie V and Dauphin-Ducharme, Philippe
Journal: Chemical communications (Cambridge, England) (2024): 6419-6422
Authors: Izadshenas Jahromi, Saeid and Słowik, Karolina
Journal: Optics letters (2024): 3914-3917
Authors: Nazeer, Shaiju S and Saraswathy, Ariya and Nimi, Nirmala and Santhakumar, Hema and Radhakrishnapillai Suma, Parvathy and Shenoy, Sachin J and Jayasree, Ramapurath S
Journal: Scientific reports (2023): 12947
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Authors: Volz, Pierre and Boreham, Alexander and Wolf, Alexander and Kim, Tai-Yang and Balke, Jens and Frombach, Janna and Hadam, Sabrina and Afraz, Zahra and Rancan, Fiorenza and Blume-Peytavi, Ulrike and Vogt, Annika and Alexiev, Ulrike
Journal: International journal of molecular sciences (2015): 6960-77