ATTO 488 DBCO

• Ex/Em: 499/520 nm
• Extinction coefficient: 90,000 cm^-1M^-1
• Reactive group: DBCO
• Copper-Free Bioorthogonal Labeling: Enables efficient azide conjugation via SPAAC without copper, ideal for sensitive systems
• Superior Brightness & Stability: Delivers consistent, intense fluorescence under demanding conditions
• High Hydrophilicity: Efficient aqueous dispersion enhances signal clarity for advanced imaging and live-cell applications

ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

The DBCO derivative of ATTO 488 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, ATTO 488 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.