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ATTO 488 DBCO
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1095.21 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.10 |
| Extinction coefficient (cm -1 M -1) | 90000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.80 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Molecular weight 1095.21 | Correction Factor (260 nm) 0.25 | Correction Factor (280 nm) 0.10 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.80 |
ATTO 488 is a rhodamine-based fluorescent dye with excellent aqueous solubility and favorable spectral properties. It exhibits strong absorption, high fluorescence quantum yield, and notable photostability, making it suitable for various fluorescence imaging applications. ATTO 488 is particularly effective in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED. It is also applicable to flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a range of other biological assays. The DBCO derivative of ATTO 488 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, ATTO 488 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ATTO 488 DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 91.307 µL | 456.533 µL | 913.067 µL | 4.565 mL | 9.131 mL |
| 5 mM | 18.261 µL | 91.307 µL | 182.613 µL | 913.067 µL | 1.826 mL |
| 10 mM | 9.131 µL | 45.653 µL | 91.307 µL | 456.533 µL | 913.067 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.10 |
| Extinction coefficient (cm -1 M -1) | 90000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.80 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ATTO 488 acid | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
| ATTO 488 maleimide | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
| ATTO 488 azide | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
| ATTO 488 alkyne | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
Images
Figure 1. ATTO 488 DBCO
References
View all 32 references: Citation Explorer
Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Analysis of photon trajectories from diffusing single molecules.
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Atto488-Agitoxin 2-A Fluorescent Ligand with Increased Selectivity for Kv1.3 Channel Binding Site.
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)
Lipophilic probe behavior in microemulsions evaluated by fluorescence correlation spectroscopy.
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
A Model of F-actin Organization in Granuloreticulopodia in Foraminifera: Morphogenetic and Evolutionary Implications from Novel Fluorescent and Polarised Light Observations.
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Evaluation of [Cys(ATTO 488)8]Dermorphin-NH2 as a novel tool for the study of μ-opioid peptide receptors.
Authors: Giakomidi, Despina and Bird, Mark F and McDonald, John and Marzola, Erika and Guerrini, Remo and Chanoch, Serena and Sabu, Nidhuna and Horley, Barbara and Calo, Girolamo and Lambert, David G
Journal: PloS one (2021): e0250011
Authors: Giakomidi, Despina and Bird, Mark F and McDonald, John and Marzola, Erika and Guerrini, Remo and Chanoch, Serena and Sabu, Nidhuna and Horley, Barbara and Calo, Girolamo and Lambert, David G
Journal: PloS one (2021): e0250011
Multimodal fluorescently labeled polymer-coated GdF3 nanoparticles inhibit degranulation in mast cells.
Authors: Shapoval, Oleksandr and Sulimenko, Vadym and Klebanovych, Anastasiya and Rabyk, Mariia and Shapoval, Pavlo and Kaman, Ondřej and Rydvalová, Eliška and Filipová, Marcela and Dráberová, Eduarda and Dráber, Pavel and Horák, Daniel
Journal: Nanoscale (2021): 19023-19037
Authors: Shapoval, Oleksandr and Sulimenko, Vadym and Klebanovych, Anastasiya and Rabyk, Mariia and Shapoval, Pavlo and Kaman, Ondřej and Rydvalová, Eliška and Filipová, Marcela and Dráberová, Eduarda and Dráber, Pavel and Horák, Daniel
Journal: Nanoscale (2021): 19023-19037