ATTO 633 TCO

ATTO 633 is a bright-red fluorescent dye, characterized by its strong absorption, high fluorescence quantum yield, and exceptional photo and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 610-645 nm wavelength range, compatible with both the 633 nm line of the He-Ne laser and the 635 nm line of the diode laser. The dye maintains stable fluorescence over a wide pH range (2-11), allowing for its use in diverse experimental conditions. Upon conjugation to a substrate, ATTO 633 becomes cationic, carrying a net positive charge of +1. These properties make ATTO 633 particularly suitable for high-precision applications, including single-molecule detection and super-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 633 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.