Calculator
BG Alkyne
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 336.36 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| BG Succinimidyl Ester |
| BG-Maleimide |
| BG Azide |
| Overview |  SDSProtocol |
Molecular weight 336.36 |
BG alkyne is an azide-reactive building block for preparing SNAP substrates from the azide-reactive forms of labels or biological targets. This BG alkyne building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications via the well-known click chemistry. AAT Bioquest offers a broad range of azide-reactive fluorescent probes and other Tag probes that can be readily coupled to BG alkyne. The SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BG Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 297.301 µL | 1.487 mL | 2.973 mL | 14.865 mL | 29.73 mL |
| 5 mM | 59.46 µL | 297.301 µL | 594.601 µL | 2.973 mL | 5.946 mL |
| 10 mM | 29.73 µL | 148.65 µL | 297.301 µL | 1.487 mL | 2.973 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. BG alkyne is an azide-reactive building block for preparing SNAP substrates from the azide-reactive forms of labels or biological targets.
References
View all 50 references: Citation Explorer
Fabrication of a bifunctional fluorescent chiral composite based on magnetic Fe3O4/chiral carbon dots@hierarchical porous metal-organic framework.
Authors: Wang, Min and Zhou, Meiling and Wang, Min and Du, Jiayin and Liu, Chunlan and Wang, Yue and Xia, Zhining
Journal: Talanta (2024): 125113
Authors: Wang, Min and Zhou, Meiling and Wang, Min and Du, Jiayin and Liu, Chunlan and Wang, Yue and Xia, Zhining
Journal: Talanta (2024): 125113
A new fluorescence-based approach for direct visualization of coat formation during sporulation in Bacillus cereus.
Authors: Lablaine, Armand and Chamot, Stéphanie and Serrano, Mónica and Billaudeau, Cyrille and Bornard, Isabelle and Carballido-López, Rut and Carlin, Frédéric and Henriques, Adriano O and Broussolle, Véronique
Journal: Scientific reports (2023): 15136
Authors: Lablaine, Armand and Chamot, Stéphanie and Serrano, Mónica and Billaudeau, Cyrille and Bornard, Isabelle and Carballido-López, Rut and Carlin, Frédéric and Henriques, Adriano O and Broussolle, Véronique
Journal: Scientific reports (2023): 15136
Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts.
Authors: Idstein, Vincent and Ehret, Anna K and Yousefi, O Sascha and Schamel, Wolfgang W
Journal: ACS synthetic biology (2023): 2857-2864
Authors: Idstein, Vincent and Ehret, Anna K and Yousefi, O Sascha and Schamel, Wolfgang W
Journal: ACS synthetic biology (2023): 2857-2864
Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters.
Authors: Zhang, Dasheng and Chen, Zhengda and Du, Zengmin and Bao, Bingkun and Su, Ni and Chen, Xianjun and Ge, Yihui and Lin, Qiuning and Yang, Lipeng and Hua, Yujie and Wang, Shuo and Hua, Xin and Zuo, Fangting and Li, Ningfeng and Liu, Renmei and Jiang, Li and Bao, Chunyan and Zhao, Yuzheng and Loscalzo, Joseph and Yang, Yi and Zhu, Linyong
Journal: Cell discovery (2023): 56
Authors: Zhang, Dasheng and Chen, Zhengda and Du, Zengmin and Bao, Bingkun and Su, Ni and Chen, Xianjun and Ge, Yihui and Lin, Qiuning and Yang, Lipeng and Hua, Yujie and Wang, Shuo and Hua, Xin and Zuo, Fangting and Li, Ningfeng and Liu, Renmei and Jiang, Li and Bao, Chunyan and Zhao, Yuzheng and Loscalzo, Joseph and Yang, Yi and Zhu, Linyong
Journal: Cell discovery (2023): 56
SNAP-Tag-Targeted MRI-Fluorescent Multimodal Probes.
Authors: Thankarajan, Ebaston and Oz, Shimrit and Saady, Abed and Kulbitski, Kseniya and Kompanets, Mykhail O and Eisen, Moris S and Berlin, Shai
Journal: Chembiochem : a European journal of chemical biology (2023): e202300172
Authors: Thankarajan, Ebaston and Oz, Shimrit and Saady, Abed and Kulbitski, Kseniya and Kompanets, Mykhail O and Eisen, Moris S and Berlin, Shai
Journal: Chembiochem : a European journal of chemical biology (2023): e202300172
Practical observations on the use of fluorescent reporter systems in Clostridioides difficile.
Authors: Oliveira Paiva, Ana M and Friggen, Annemieke H and Douwes, Roxanne and Wittekoek, Bert and Smits, Wiep Klaas
Journal: Antonie van Leeuwenhoek (2022): 297-323
Authors: Oliveira Paiva, Ana M and Friggen, Annemieke H and Douwes, Roxanne and Wittekoek, Bert and Smits, Wiep Klaas
Journal: Antonie van Leeuwenhoek (2022): 297-323
A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins.
Authors: Baek, Inwha and Le, Sarah N and Jeon, Jongcheol and Chun, Yujin and Reed, Charlotte and Buratowski, Stephen
Journal: G3 (Bethesda, Md.) (2022)
Authors: Baek, Inwha and Le, Sarah N and Jeon, Jongcheol and Chun, Yujin and Reed, Charlotte and Buratowski, Stephen
Journal: G3 (Bethesda, Md.) (2022)
Super-Resolution Imaging of Autophagy by a Preferred Pair of Self-Labeling Protein Tags and Fluorescent Ligands.
Authors: Man, Huizi and Zhou, Lin and Zhu, Guanghao and Zheng, Ying and Ye, Zhiwei and Huang, Zhenlong and Teng, Xinru and Ai, Chunzhi and Ge, Guangbo and Xiao, Yi
Journal: Analytical chemistry (2022): 15057-15066
Authors: Man, Huizi and Zhou, Lin and Zhu, Guanghao and Zheng, Ying and Ye, Zhiwei and Huang, Zhenlong and Teng, Xinru and Ai, Chunzhi and Ge, Guangbo and Xiao, Yi
Journal: Analytical chemistry (2022): 15057-15066
Fluorescent pulse-chase labeling to monitor long-term mitochondrial degradation in primary hippocampal neurons.
Authors: Schneider, Jordan R and Evans, Chantell S
Journal: STAR protocols (2022): 101822
Authors: Schneider, Jordan R and Evans, Chantell S
Journal: STAR protocols (2022): 101822
N-Methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy.
Authors: Roßmann, Kilian and Akkaya, Kerem C and Poc, Pascal and Charbonnier, Corentin and Eichhorst, Jenny and Gonschior, Hannes and Valavalkar, Abha and Wendler, Nicolas and Cordes, Thorben and Dietzek-Ivanšić, Benjamin and Jones, Ben and Lehmann, Martin and Broichhagen, Johannes
Journal: Chemical science (2022): 8605-8617
Authors: Roßmann, Kilian and Akkaya, Kerem C and Poc, Pascal and Charbonnier, Corentin and Eichhorst, Jenny and Gonschior, Hannes and Valavalkar, Abha and Wendler, Nicolas and Cordes, Thorben and Dietzek-Ivanšić, Benjamin and Jones, Ben and Lehmann, Martin and Broichhagen, Johannes
Journal: Chemical science (2022): 8605-8617