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BG Alkyne
BG alkyne is an azide-reactive building block for preparing SNAP substrates from the azide-reactive forms of labels or biological targets. This BG alkyne building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications via the well-known click chemistry. AAT Bioquest offers a broad range of azide-reactive fluorescent probes and other Tag probes that can be readily coupled to BG alkyne. The SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| AMCA Alkyne | 346 | 434 | 19000 | 0.153 |
| XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
| ICG alkyne | 789 | 813 | 230000 | 0.076 |
| Cy3B alkyne | 560 | 571 | 1200001 | 0.069 |
| XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
| XFD350 alkyne | 343 | 441 | 19000 | 0.19 |
| XFD405 alkyne | 401 | 421 | 35,000 | 0.70 |
| XFD430 alkyne | 432 | 540 | 15,000 | 0.28 |
| XFD514 alkyne | 518 | 543 | 80000 | 0.18 |
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References
View all 50 references: Citation Explorer
Fabrication of a bifunctional fluorescent chiral composite based on magnetic Fe3O4/chiral carbon dots@hierarchical porous metal-organic framework.
Authors: Wang, Min and Zhou, Meiling and Wang, Min and Du, Jiayin and Liu, Chunlan and Wang, Yue and Xia, Zhining
Journal: Talanta (2024): 125113
Authors: Wang, Min and Zhou, Meiling and Wang, Min and Du, Jiayin and Liu, Chunlan and Wang, Yue and Xia, Zhining
Journal: Talanta (2024): 125113
A new fluorescence-based approach for direct visualization of coat formation during sporulation in Bacillus cereus.
Authors: Lablaine, Armand and Chamot, Stéphanie and Serrano, Mónica and Billaudeau, Cyrille and Bornard, Isabelle and Carballido-López, Rut and Carlin, Frédéric and Henriques, Adriano O and Broussolle, Véronique
Journal: Scientific reports (2023): 15136
Authors: Lablaine, Armand and Chamot, Stéphanie and Serrano, Mónica and Billaudeau, Cyrille and Bornard, Isabelle and Carballido-López, Rut and Carlin, Frédéric and Henriques, Adriano O and Broussolle, Véronique
Journal: Scientific reports (2023): 15136
Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts.
Authors: Idstein, Vincent and Ehret, Anna K and Yousefi, O Sascha and Schamel, Wolfgang W
Journal: ACS synthetic biology (2023): 2857-2864
Authors: Idstein, Vincent and Ehret, Anna K and Yousefi, O Sascha and Schamel, Wolfgang W
Journal: ACS synthetic biology (2023): 2857-2864
Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters.
Authors: Zhang, Dasheng and Chen, Zhengda and Du, Zengmin and Bao, Bingkun and Su, Ni and Chen, Xianjun and Ge, Yihui and Lin, Qiuning and Yang, Lipeng and Hua, Yujie and Wang, Shuo and Hua, Xin and Zuo, Fangting and Li, Ningfeng and Liu, Renmei and Jiang, Li and Bao, Chunyan and Zhao, Yuzheng and Loscalzo, Joseph and Yang, Yi and Zhu, Linyong
Journal: Cell discovery (2023): 56
Authors: Zhang, Dasheng and Chen, Zhengda and Du, Zengmin and Bao, Bingkun and Su, Ni and Chen, Xianjun and Ge, Yihui and Lin, Qiuning and Yang, Lipeng and Hua, Yujie and Wang, Shuo and Hua, Xin and Zuo, Fangting and Li, Ningfeng and Liu, Renmei and Jiang, Li and Bao, Chunyan and Zhao, Yuzheng and Loscalzo, Joseph and Yang, Yi and Zhu, Linyong
Journal: Cell discovery (2023): 56
SNAP-Tag-Targeted MRI-Fluorescent Multimodal Probes.
Authors: Thankarajan, Ebaston and Oz, Shimrit and Saady, Abed and Kulbitski, Kseniya and Kompanets, Mykhail O and Eisen, Moris S and Berlin, Shai
Journal: Chembiochem : a European journal of chemical biology (2023): e202300172
Authors: Thankarajan, Ebaston and Oz, Shimrit and Saady, Abed and Kulbitski, Kseniya and Kompanets, Mykhail O and Eisen, Moris S and Berlin, Shai
Journal: Chembiochem : a European journal of chemical biology (2023): e202300172
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