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AAT Bioquest

BG Azide

BG azide is an alkyne-reactive building block for preparing SNAP substrates from the alkyne-reactive forms of labels or biological targets. This BG azide building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications via the well-known click chemistry. AAT Bioquest offers a broad range of alkyne-reactive fluorescent probes and other Tag probes that can be readily coupled to BG azide. The SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
AMCA Azide346434190000.153
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*499520710000.11
ICG azide7898132300000.076
Cy3B azide56057112000010.069
XFD647 Azide6506712700000.03
XFD350 azide343441190000.19
XFD405 azide40142135,0000.70
XFD430 azide43254015,0000.28
XFD514 azide518543800000.18
XFD546 azide5615721120000.12
XFD532 azide534553810000.09
XFD568 azide579603913000.46
XFD594 azide590618900000.56
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References

View all 50 references: Citation Explorer
Revealing receptor-ligand interactions of atypical antipsychotic drugs and screening anti-schizophrenia ingredients in Magnolia officinalis based on 5-HTR2A-SNAP-Tag/CMC and DRD2-SNAP-Tag/CMC models.
Authors: Gao, Chunlei and Zhou, Tongpei and Liu, Sihan and Miao, Chenyang and Feng, Jingting and Ding, Yifan and Lv, Yanni
Journal: Journal of chromatography. A (2024): 464784
KIT-SNAP-tag/cell membrane chromatography model coupled with liquid chromatography-mass spectrometry for anti-GIST compound screening from Evodia rutaecarpa.
Authors: Feng, Jingting and Jiang, Yuhan and Liu, Sihan and Deng, Linge and Lv, Yanni and Chen, Nanzheng and Han, Shengli
Journal: Analytical and bioanalytical chemistry (2024): 1457-1468
Mapping histone variant genomic distribution: Exploiting SNAP-tag labeling to follow the dynamics of incorporation of H3 variants.
Authors: Forest, Audrey and Quivy, Jean-Pierre and Almouzni, Geneviève
Journal: Methods in cell biology (2024): 49-65
Application of SNAP-tag-EGFR cell membrane chromatography model in screening antitumor active components of Silybum marianum (L.) Gaertn.
Authors: Zhang, Xin and Jiang, Yuhan and Guo, Na and Ding, Yifan and Feng, Jingting and Miao, Chenyang and Lv, Yanni
Journal: Journal of pharmaceutical and biomedical analysis (2024): 115816
The Evolution of SNAP-Tag Labels.
Authors: Dreyer, Rudolf and Pfukwa, Rueben and Barth, Stefan and Hunter, Roger and Klumperman, Bert
Journal: Biomacromolecules (2023): 517-530
Page updated on November 23, 2024

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Catalog Number12568
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Physical properties

Molecular weight

381.40

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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