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BG Azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 381.40 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| BG Succinimidyl Ester |
| BG-Maleimide |
| BG Alkyne |
| Overview |  SDSProtocol |
Molecular weight 381.40 |
BG azide is an alkyne-reactive building block for preparing SNAP substrates from the alkyne-reactive forms of labels or biological targets. This BG azide building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications via the well-known click chemistry. AAT Bioquest offers a broad range of alkyne-reactive fluorescent probes and other Tag probes that can be readily coupled to BG azide. The SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BG Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 262.192 µL | 1.311 mL | 2.622 mL | 13.11 mL | 26.219 mL |
| 5 mM | 52.438 µL | 262.192 µL | 524.384 µL | 2.622 mL | 5.244 mL |
| 10 mM | 26.219 µL | 131.096 µL | 262.192 µL | 1.311 mL | 2.622 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. BG azide is an alkyne-reactive building block for preparing SNAP substrates from the alkyne-reactive forms of labels or biological targets.
References
View all 50 references: Citation Explorer
Revealing receptor-ligand interactions of atypical antipsychotic drugs and screening anti-schizophrenia ingredients in Magnolia officinalis based on 5-HTR2A-SNAP-Tag/CMC and DRD2-SNAP-Tag/CMC models.
Authors: Gao, Chunlei and Zhou, Tongpei and Liu, Sihan and Miao, Chenyang and Feng, Jingting and Ding, Yifan and Lv, Yanni
Journal: Journal of chromatography. A (2024): 464784
Authors: Gao, Chunlei and Zhou, Tongpei and Liu, Sihan and Miao, Chenyang and Feng, Jingting and Ding, Yifan and Lv, Yanni
Journal: Journal of chromatography. A (2024): 464784
KIT-SNAP-tag/cell membrane chromatography model coupled with liquid chromatography-mass spectrometry for anti-GIST compound screening from Evodia rutaecarpa.
Authors: Feng, Jingting and Jiang, Yuhan and Liu, Sihan and Deng, Linge and Lv, Yanni and Chen, Nanzheng and Han, Shengli
Journal: Analytical and bioanalytical chemistry (2024): 1457-1468
Authors: Feng, Jingting and Jiang, Yuhan and Liu, Sihan and Deng, Linge and Lv, Yanni and Chen, Nanzheng and Han, Shengli
Journal: Analytical and bioanalytical chemistry (2024): 1457-1468
Mapping histone variant genomic distribution: Exploiting SNAP-tag labeling to follow the dynamics of incorporation of H3 variants.
Authors: Forest, Audrey and Quivy, Jean-Pierre and Almouzni, Geneviève
Journal: Methods in cell biology (2024): 49-65
Authors: Forest, Audrey and Quivy, Jean-Pierre and Almouzni, Geneviève
Journal: Methods in cell biology (2024): 49-65
Application of SNAP-tag-EGFR cell membrane chromatography model in screening antitumor active components of Silybum marianum (L.) Gaertn.
Authors: Zhang, Xin and Jiang, Yuhan and Guo, Na and Ding, Yifan and Feng, Jingting and Miao, Chenyang and Lv, Yanni
Journal: Journal of pharmaceutical and biomedical analysis (2024): 115816
Authors: Zhang, Xin and Jiang, Yuhan and Guo, Na and Ding, Yifan and Feng, Jingting and Miao, Chenyang and Lv, Yanni
Journal: Journal of pharmaceutical and biomedical analysis (2024): 115816
The Evolution of SNAP-Tag Labels.
Authors: Dreyer, Rudolf and Pfukwa, Rueben and Barth, Stefan and Hunter, Roger and Klumperman, Bert
Journal: Biomacromolecules (2023): 517-530
Authors: Dreyer, Rudolf and Pfukwa, Rueben and Barth, Stefan and Hunter, Roger and Klumperman, Bert
Journal: Biomacromolecules (2023): 517-530
Click Chemistry-Generated Auristatin F-Linker-Benzylguanine for a SNAP-Tag-Based Recombinant Antibody-Drug Conjugate Demonstrating Selective Cytotoxicity toward EGFR-Overexpressing Tumor Cells.
Authors: Huysamen, Allan M and Fadeyi, Olaolu E and Mayuni, Grace and Dogbey, Dennis M and Mungra, Neelakshi and Biteghe, Fleury A N and Hardcastle, Natasha and Ramamurthy, Dharanidharan and Akinrinmade, Olusiji A and Naran, Krupa and Cooper, Susan and Lang, Dirk and Richter, Wolfgang and Hunter, Roger and Barth, Stefan
Journal: ACS omega (2023): 4026-4037
Authors: Huysamen, Allan M and Fadeyi, Olaolu E and Mayuni, Grace and Dogbey, Dennis M and Mungra, Neelakshi and Biteghe, Fleury A N and Hardcastle, Natasha and Ramamurthy, Dharanidharan and Akinrinmade, Olusiji A and Naran, Krupa and Cooper, Susan and Lang, Dirk and Richter, Wolfgang and Hunter, Roger and Barth, Stefan
Journal: ACS omega (2023): 4026-4037
Establishment of angiotensin-converting enzyme 2 and cluster of differentiation 147 dual target cell membrane chromatography based on SNAP-tag technology for screening anti severe acute respiratory syndrome coronavirus 2 active components.
Authors: Wang, Hong and Jia, Qianqian and Feng, Jingting and Miao, Chenyang and Ding, Yifan and Liu, Sihan and Feng, Chaohua and Lv, Yanni and Huang, Jing and Han, Shengli
Journal: Journal of chromatography. A (2023): 463903
Authors: Wang, Hong and Jia, Qianqian and Feng, Jingting and Miao, Chenyang and Ding, Yifan and Liu, Sihan and Feng, Chaohua and Lv, Yanni and Huang, Jing and Han, Shengli
Journal: Journal of chromatography. A (2023): 463903
CSPG4 as a target for the specific killing of triple-negative breast cancer cells by a recombinant SNAP-tag-based antibody-auristatin F drug conjugate.
Authors: Mungra, Neelakshi and Biteghe, Fleury A N and Malindi, Zaria and Huysamen, Allan M and Karaan, Maryam and Hardcastle, Natasha S and Bunjun, Rubina and Chetty, Shivan and Naran, Krupa and Lang, Dirk and Richter, Wolfgang and Hunter, Roger and Barth, Stefan
Journal: Journal of cancer research and clinical oncology (2023): 12203-12225
Authors: Mungra, Neelakshi and Biteghe, Fleury A N and Malindi, Zaria and Huysamen, Allan M and Karaan, Maryam and Hardcastle, Natasha S and Bunjun, Rubina and Chetty, Shivan and Naran, Krupa and Lang, Dirk and Richter, Wolfgang and Hunter, Roger and Barth, Stefan
Journal: Journal of cancer research and clinical oncology (2023): 12203-12225
Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters.
Authors: Zhang, Dasheng and Chen, Zhengda and Du, Zengmin and Bao, Bingkun and Su, Ni and Chen, Xianjun and Ge, Yihui and Lin, Qiuning and Yang, Lipeng and Hua, Yujie and Wang, Shuo and Hua, Xin and Zuo, Fangting and Li, Ningfeng and Liu, Renmei and Jiang, Li and Bao, Chunyan and Zhao, Yuzheng and Loscalzo, Joseph and Yang, Yi and Zhu, Linyong
Journal: Cell discovery (2023): 56
Authors: Zhang, Dasheng and Chen, Zhengda and Du, Zengmin and Bao, Bingkun and Su, Ni and Chen, Xianjun and Ge, Yihui and Lin, Qiuning and Yang, Lipeng and Hua, Yujie and Wang, Shuo and Hua, Xin and Zuo, Fangting and Li, Ningfeng and Liu, Renmei and Jiang, Li and Bao, Chunyan and Zhao, Yuzheng and Loscalzo, Joseph and Yang, Yi and Zhu, Linyong
Journal: Cell discovery (2023): 56
SNAP-Tag-Targeted MRI-Fluorescent Multimodal Probes.
Authors: Thankarajan, Ebaston and Oz, Shimrit and Saady, Abed and Kulbitski, Kseniya and Kompanets, Mykhail O and Eisen, Moris S and Berlin, Shai
Journal: Chembiochem : a European journal of chemical biology (2023): e202300172
Authors: Thankarajan, Ebaston and Oz, Shimrit and Saady, Abed and Kulbitski, Kseniya and Kompanets, Mykhail O and Eisen, Moris S and Berlin, Shai
Journal: Chembiochem : a European journal of chemical biology (2023): e202300172