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AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA acid *CAS#: 106562-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 428.78 µL | 2.144 mL | 4.288 mL | 21.439 mL | 42.878 mL |
| 5 mM | 85.756 µL | 428.78 µL | 857.559 µL | 4.288 mL | 8.576 mL |
| 10 mM | 42.878 µL | 214.39 µL | 428.78 µL | 2.144 mL | 4.288 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| ICG acid | 789 | 813 | 230000 | 0.076 |
| Cy3NS acid *CAS 1032678-01-5* | 555 | 569 | 1500001 | 0.073 |
| Cy5NS acid | 651 | 670 | 2500001 | 0.03 |
| Cy7NS acid | 756 | 779 | 250000 | 0.036 |
| AMCA Ethylenediamine | 346 | 434 | 19000 | 0.153 |
| AMCA Alkyne | 346 | 434 | 19000 | 0.153 |
| AMCA Azide | 346 | 434 | 19000 | 0.153 |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7* | 336 | 455 | 5900 | 0.107 |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] | 336 | 455 | 5900 | 0.107 |
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References
View all 13 references: Citation Explorer
Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1
Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160
Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518
Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240
Oral surgical procedures during anticoagulant therapy
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681
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