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DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*
DABCYL acid is one of the most popular acceptors for developing FRET-based nucleic acid probes suh as Molecular Beacon-based PCR probes. It is also used to prepare a variety of FRET peptides that are often used for the detection of various proteases. It is predominantly paired with EDANS (as fluorescence donor) in FRET-based fluorescent probes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 371.333 µL | 1.857 mL | 3.713 mL | 18.567 mL | 37.133 mL |
| 5 mM | 74.267 µL | 371.333 µL | 742.666 µL | 3.713 mL | 7.427 mL |
| 10 mM | 37.133 µL | 185.667 µL | 371.333 µL | 1.857 mL | 3.713 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) | Correction Factor (260 nm) |
| ICG acid | 789 | 813 | 230000 | 0.076 | - |
| Cy3NS acid *CAS 1032678-01-5* | 555 | 569 | 1500001 | 0.073 | 0.07 |
| Cy5NS acid | 651 | 670 | 2500001 | 0.03 | 0.02 |
| Cy7NS acid | 756 | 779 | 250000 | 0.036 | 0.05 |
| AMCA acid *CAS#: 106562-32-7* | 346 | 434 | 19000 | 0.153 | - |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7* | 336 | 455 | 5900 | 0.107 | - |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] | 336 | 455 | 5900 | 0.107 | - |
| BCECF acid *CAS#: 85138-49-4* | 504 | 527 | 90000 | - | - |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.09 | 0.24 |
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Citations
View all 3 citations: Citation Explorer
Peptide Based Inhibitors of Protein binding to the Mitogen-Activated Protein Kinase Docking Groove
Authors: Alexa, Anita and Ember, Orsolya and Szab{\'o}, Ildik{\'o} and Mo'ath, Yousef and P{\'o}ti, {\'A}d{\'a}m L and Rem{\'e}nyi, Attila and B{\'a}n{\'o}czi, Zolt{\'a}n
Journal: Frontiers in molecular biosciences (2021): 629
Authors: Alexa, Anita and Ember, Orsolya and Szab{\'o}, Ildik{\'o} and Mo'ath, Yousef and P{\'o}ti, {\'A}d{\'a}m L and Rem{\'e}nyi, Attila and B{\'a}n{\'o}czi, Zolt{\'a}n
Journal: Frontiers in molecular biosciences (2021): 629
Influence of the Dabcyl group on the cellular uptake of cationic peptides: short oligoarginines as efficient cell-penetrating peptides
Authors: Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,
Journal: Amino Acids (2021): 1--17
Authors: Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,
Journal: Amino Acids (2021): 1--17
Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599
References
View all 34 references: Citation Explorer
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
ADAM33 enzyme properties and substrate specificity
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247
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