XFD430 acid

• Ex/Em: 432/540 nm
• Extinction coefficient: 15,000 cm^-1M^-1
• pH-Insensitive & High Hydrophilicity: Maintains stable fluorescence across pH 4–10 and minimizes aggregation
• Large Stokes Shift: Provides clear signal separation and minimizes spectral overlap, enhancing imaging accuracy in sensitive applications

XFD430, manufactured by AAT Bioquest, is a green-fluorescent dye that is structurally identical to Alexa Fluor™ 430 (ThermoFisher). It has excitation and emission maxima of 432 nm and 540 nm, respectively, and is efficiently excited by a 405 nm violet laser. Its large Stokes shift minimizes spectral overlap with blue-emitting fluorophores, significantly improving the signal-to-noise ratio in multiplexed fluorescence assays. XFD430 also exhibits excellent water solubility and remains pH-insensitive across a wide range (pH 4–10), making it well-suited for generating stable, reproducible fluorescence signals in both microscopy and flow cytometry applications.

XFD430 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD430 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.