XFD635 azide

• Ex/Em: 633/647 nm
• Extinction Coefficient: 140,000 cm^-1M^-1
• Reactive Group: Azide
• Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
• Advanced Dye Properties: Excellent water solubility and structural match to Alexa Fluor™ 635
• Robust Signal Performance: Maintains high intensity and pH independence (pH 4-11) with exceptional photostability

XFD635, manufactured by AAT Bioquest, is a bright far red-fluorescent dye structurally identical to Alexa Fluor™ 635 (ThermoFisher). It is characterized by its intense brightness, high fluorescence quantum yield and photostability. The dye demonstrates excellent solubility in aqueous solutions and retains pH-independent fluorescence over a broad range (pH 4–11), ensuring consistent performance across diverse experimental conditions. Optimally excited by the 633 nm emission line of He-Ne lasers or the 635 nm diode laser, XFD635 is particularly well-suited for flow cytometry. Its robust and uniform labeling properties yield high signal intensity and reproducibility, making it an ideal choice for advanced fluorescence imaging, flow cytometry, and various fluorescence-based analytical techniques.

The azide derivative of XFD635 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.