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NTA Azide
Nitrilotriacetic acid (NTA) is a well-knows ligand commonly used in commercially available resins for purifying proteins. On the other hand, tag-NTA conjugates are used for detecting polyhistidine-tagged proteins. NTA azide is an excellent alkyne-reactive building block that can be readily used to attach NTA groups to a biological substrate or a surface via the well-known click chemistry. Through the NTA moiety attached to the substrate or surface, genetically expressed proteins bearing the hexahistidine extension (HIS Tag) can be detected or immobilized via Ni-mediated His-Tag method. Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| AMCA Azide | 346 | 434 | 19000 | 0.153 |
| XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.11 |
| ICG azide | 789 | 813 | 230000 | 0.076 |
| Cy3B azide | 560 | 571 | 1200001 | 0.069 |
| XFD647 Azide | 650 | 671 | 270000 | 0.03 |
| XFD350 azide | 343 | 441 | 19000 | 0.19 |
| XFD405 azide | 401 | 421 | 35,000 | 0.70 |
| XFD430 azide | 432 | 540 | 15,000 | 0.28 |
| XFD514 azide | 518 | 543 | 80000 | 0.18 |
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References
View all 50 references: Citation Explorer
Molecular cloning, characterization and expression analysis of the Chinese soft-shelled turtle (Pelodiscus sinensis) chemokine CXCL11.
Authors: Zhang, Airu and Ding, Yujie and Huang, Qiuya and Qian, Guoying and Munang'andu, Hetron Mweemba and Xu, Cheng and Xu, Jiehao
Journal: Fish & shellfish immunology (2024): 109331
Authors: Zhang, Airu and Ding, Yujie and Huang, Qiuya and Qian, Guoying and Munang'andu, Hetron Mweemba and Xu, Cheng and Xu, Jiehao
Journal: Fish & shellfish immunology (2024): 109331
Administration sequence- and formation-dependent vaccination using acid-degradable polymeric nanoparticles with high antigen encapsulation capability.
Authors: Choi, Yeon Su and Felgner, Jiin and Jan, Sharon and Hernandez-Davies, Jenny E and Davies, D Huw and Kwon, Young Jik
Journal: Journal of materials chemistry. B (2024): 6577-6586
Authors: Choi, Yeon Su and Felgner, Jiin and Jan, Sharon and Hernandez-Davies, Jenny E and Davies, D Huw and Kwon, Young Jik
Journal: Journal of materials chemistry. B (2024): 6577-6586
Histidine tag modified magnetic beads for analysis of arsenic binding proteins.
Authors: Nan, Kai and He, Man and Chen, Beibei and Hu, Bin
Journal: Analytica chimica acta (2024): 342554
Authors: Nan, Kai and He, Man and Chen, Beibei and Hu, Bin
Journal: Analytica chimica acta (2024): 342554
Prokaryotic Expression and Affinity Purification of DDX3 Protein.
Authors: Huang, Lan and Liang, Yue and Hou, Huijin and Tang, Min and Liu, Xinpeng and Ma, Yan-Ni and Liang, Shufang
Journal: Protein and peptide letters (2024): 236-246
Authors: Huang, Lan and Liang, Yue and Hou, Huijin and Tang, Min and Liu, Xinpeng and Ma, Yan-Ni and Liang, Shufang
Journal: Protein and peptide letters (2024): 236-246
Preparation, Characterization, and Radiolabeling of Anti-HER2 scFv With Technetium Tricarbonyl and Stability Studies.
Authors: Bozorgchami, Negar and Ahmadzadeh, Maryam and Hatamabadi, Dara and Yazdani, Abdolreza and Shahhosseini, Soraya and Mohit, Elham
Journal: Journal of labelled compounds & radiopharmaceuticals (2024): 168-179
Authors: Bozorgchami, Negar and Ahmadzadeh, Maryam and Hatamabadi, Dara and Yazdani, Abdolreza and Shahhosseini, Soraya and Mohit, Elham
Journal: Journal of labelled compounds & radiopharmaceuticals (2024): 168-179
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