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NTA Alkyne
Nitrilotriacetic acid (NTA) is a well-knows ligand commonly used in commercially available resins for purifying proteins. On the other hand, tag-NTA conjugates are used for detecting polyhistidine-tagged proteins. NTA alkyne is an excellent azido-reactive building block that can be readily used to attach NTA groups to a biological substrate or a surface via the well-known click chemistry. Through the NTA moiety attached to the substrate or surface, genetically expressed proteins bearing the hexahistidine extension (HIS Tag) can be detected or immobilized via Ni-mediated His-Tag method. Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| AMCA Alkyne | 346 | 434 | 19000 | 0.153 |
| XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
| ICG alkyne | 789 | 813 | 230000 | 0.076 |
| Cy3B alkyne | 560 | 571 | 1200001 | 0.069 |
| XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
| XFD350 alkyne | 343 | 441 | 19000 | 0.19 |
| XFD405 alkyne | 401 | 421 | 35,000 | 0.70 |
| XFD430 alkyne | 432 | 540 | 15,000 | 0.28 |
| XFD514 alkyne | 518 | 543 | 80000 | 0.18 |
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References
View all 50 references: Citation Explorer
Construction of a highly efficient adsorbent for one-step purification of recombinant proteins: Functionalized cellulose-based monolith fabricated via phase separation method.
Authors: Huang, Lanlan and Chen, Guronghua and Zhang, Guozhi and Fang, Yue and Zhu, Wenjie and Xin, Yuanrong
Journal: Carbohydrate polymers (2024): 122046
Authors: Huang, Lanlan and Chen, Guronghua and Zhang, Guozhi and Fang, Yue and Zhu, Wenjie and Xin, Yuanrong
Journal: Carbohydrate polymers (2024): 122046
Tumor-specific targeting of polymer drug delivery systems with recombinant proteins bound via tris(nitrilotriacetic acid).
Authors: Pechar, Michal and Král, Vlastimil and Kracíková, Lucie and Androvič, Ladislav and Hrdá, Eliška and Pola, Robert and Pytlíková, Sára and Studenovský, Martin and Kostka, Libor and Šubr, Vladimír and Etrych, Tomáš and Kočková, Olga and Marianne Ferreira Mendes, Jessica and Fábry, Milan and Laga, Richard
Journal: International journal of pharmaceutics (2023): 123619
Authors: Pechar, Michal and Král, Vlastimil and Kracíková, Lucie and Androvič, Ladislav and Hrdá, Eliška and Pola, Robert and Pytlíková, Sára and Studenovský, Martin and Kostka, Libor and Šubr, Vladimír and Etrych, Tomáš and Kočková, Olga and Marianne Ferreira Mendes, Jessica and Fábry, Milan and Laga, Richard
Journal: International journal of pharmaceutics (2023): 123619
Streptavidin-Conjugated DNA for the Boronate Affinity-Based Detection of Poly(ADP-Ribose) Polymerase-1 with Improved Sensitivity.
Authors: Gao, Fengli and Liu, Gang and Qiao, Yishu and Dong, Xiuwen and Liu, Lin
Journal: Biosensors (2023)
Authors: Gao, Fengli and Liu, Gang and Qiao, Yishu and Dong, Xiuwen and Liu, Lin
Journal: Biosensors (2023)
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites.
Authors: Khataminezhad, Ehteram Sadat and Hajihassan, Zahra and Razi Astaraei, Fatemeh
Journal: Protein expression and purification (2023): 106264
Authors: Khataminezhad, Ehteram Sadat and Hajihassan, Zahra and Razi Astaraei, Fatemeh
Journal: Protein expression and purification (2023): 106264
A Simple and Versatile Strategy for Oriented Immobilization of His-Tagged Proteins on Magnetic Nanoparticles.
Authors: Castro-Hinojosa, Christian and Del Sol-Fernández, Susel and Moreno-Antolín, Eduardo and Martín-Gracia, Beatriz and Ovejero, Jesús G and de la Fuente, Jesús Martínez and Grazú, Valeria and Fratila, Raluca M and Moros, María
Journal: Bioconjugate chemistry (2023)
Authors: Castro-Hinojosa, Christian and Del Sol-Fernández, Susel and Moreno-Antolín, Eduardo and Martín-Gracia, Beatriz and Ovejero, Jesús G and de la Fuente, Jesús Martínez and Grazú, Valeria and Fratila, Raluca M and Moros, María
Journal: Bioconjugate chemistry (2023)
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