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AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in TE Buffer (pH 7.5)* *CAS 936327-10-5*

The amine-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminoallyl dUTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminoallyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled uridine triphosphates that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
TF1-dUTP *1 mM in TE Buffer (pH 7.5)*42046135000-
TF2-dUTP *1 mM in TE Buffer (pH 7.5)*503525750000.09
TF3-dUTP *1 mM in TE Buffer (pH 7.5)*5535787500010.179
TF4-dUTP *1 mM in TE Buffer (pH 7.5)*577602900000.436
TF5-dUTP *1 mM in TE Buffer (pH 7.5)*6496632500000.027
Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)*552578900000.178
DEAC-dUTP *1 mM in TE Buffer (pH 7.5)*411472-0.12

References

View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Page updated on November 12, 2024

Ordering information

Price
Unit size
1 umole
2.5 umoles
Catalog Number
1700417005
Quantity
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Physical properties

Molecular weight

589.17

Solvent

Water

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134

CAS

936327-10-5
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