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TF5-dUTP *1 mM in TE Buffer (pH 7.5)*

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TF5-dUTP conjugate can be used as a deep red fluorescence color with Cy5® filter set. (Cy5® is the trademark of GE Healthcare).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
TF1-dUTP *1 mM in TE Buffer (pH 7.5)*42046135000-
TF2-dUTP *1 mM in TE Buffer (pH 7.5)*503525750000.09
TF3-dUTP *1 mM in TE Buffer (pH 7.5)*5535787500010.179
TF4-dUTP *1 mM in TE Buffer (pH 7.5)*577602900000.436
Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)*552578900000.178
DEAC-dUTP *1 mM in TE Buffer (pH 7.5)*411472-0.12

References

View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Page updated on November 20, 2024

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Catalog Number17010
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Physical properties

Molecular weight

~1570

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.027

Extinction coefficient (cm -1 M -1)

250000

Excitation (nm)

649

Emission (nm)

663

Quantum yield

0.271

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134
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