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TF1-dUTP *1 mM in TE Buffer (pH 7.5)*

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TF1-dUTP conjugate can be used as a blue fluorescence color with Spectrum Aqua™ filter set. (Spectrum Aqua™ is the trademark of Vysis).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of TF1-dUTP *1 mM in TE Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM107.347 µL536.734 µL1.073 mL5.367 mL10.735 mL
5 mM21.469 µL107.347 µL214.694 µL1.073 mL2.147 mL
10 mM10.735 µL53.673 µL107.347 µL536.734 µL1.073 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
TF2-dUTP *1 mM in TE Buffer (pH 7.5)*503525750000.910.09
TF3-dUTP *1 mM in TE Buffer (pH 7.5)*5535787500010.110.179
TF4-dUTP *1 mM in TE Buffer (pH 7.5)*577602900000.7710.436
TF5-dUTP *1 mM in TE Buffer (pH 7.5)*6496632500000.2710.027
Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)*55257890000-0.178
DEAC-dUTP *1 mM in TE Buffer (pH 7.5)*411472--0.12

References

View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Page updated on November 12, 2024

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Catalog Number17006
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Physical properties

Molecular weight

931.56

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

35000

Excitation (nm)

420

Emission (nm)

461

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134
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