Calculator
TF1-dUTP *1 mM in TE Buffer (pH 7.5)*
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TF1-dUTP conjugate can be used as a blue fluorescence color with Spectrum Aqua™ filter set. (Spectrum Aqua™ is the trademark of Vysis).
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of TF1-dUTP *1 mM in TE Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 107.347 µL | 536.734 µL | 1.073 mL | 5.367 mL | 10.735 mL |
| 5 mM | 21.469 µL | 107.347 µL | 214.694 µL | 1.073 mL | 2.147 mL |
| 10 mM | 10.735 µL | 53.673 µL | 107.347 µL | 536.734 µL | 1.073 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| TF2-dUTP *1 mM in TE Buffer (pH 7.5)* | 503 | 525 | 75000 | 0.91 | 0.09 |
| TF3-dUTP *1 mM in TE Buffer (pH 7.5)* | 553 | 578 | 750001 | 0.11 | 0.179 |
| TF4-dUTP *1 mM in TE Buffer (pH 7.5)* | 577 | 602 | 90000 | 0.771 | 0.436 |
| TF5-dUTP *1 mM in TE Buffer (pH 7.5)* | 649 | 663 | 250000 | 0.271 | 0.027 |
| Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)* | 552 | 578 | 90000 | - | 0.178 |
| DEAC-dUTP *1 mM in TE Buffer (pH 7.5)* | 411 | 472 | - | - | 0.12 |
References
View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
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