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Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)*
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TMR-dUTP conjugate can be used as an orange fluorescence color with Spectrum Orange™ filter set (Spectrum Orange™ is the trademark of Vysis).
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| TF1-dUTP *1 mM in TE Buffer (pH 7.5)* | 420 | 461 | 35000 | - | - |
| TF2-dUTP *1 mM in TE Buffer (pH 7.5)* | 503 | 525 | 75000 | 0.91 | 0.09 |
| TF3-dUTP *1 mM in TE Buffer (pH 7.5)* | 553 | 578 | 750001 | 0.11 | 0.179 |
| TF4-dUTP *1 mM in TE Buffer (pH 7.5)* | 577 | 602 | 90000 | 0.771 | 0.436 |
| TF5-dUTP *1 mM in TE Buffer (pH 7.5)* | 649 | 663 | 250000 | 0.271 | 0.027 |
| DEAC-dUTP *1 mM in TE Buffer (pH 7.5)* | 411 | 472 | - | - | 0.12 |
References
View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
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