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DEAC-dUTP *1 mM in TE Buffer (pH 7.5)*
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Coumarin-dUTP conjugate can be used as a blue fluorescence color with Spectrum Aqua™ filter set (Spectrum Aqua™ is the trademark of Vysis). It is chemically same to Diethylaminocoumarin-5-dUTP (Perkin Elmer’s NEL455001EA).
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] *CAS 139346-57-9* | 411 | 472 | - | 0.12 |
| TF1-dUTP *1 mM in TE Buffer (pH 7.5)* | 420 | 461 | 35000 | - |
| TF2-dUTP *1 mM in TE Buffer (pH 7.5)* | 503 | 525 | 75000 | 0.09 |
| TF3-dUTP *1 mM in TE Buffer (pH 7.5)* | 553 | 578 | 750001 | 0.179 |
| TF4-dUTP *1 mM in TE Buffer (pH 7.5)* | 577 | 602 | 90000 | 0.436 |
| TF5-dUTP *1 mM in TE Buffer (pH 7.5)* | 649 | 663 | 250000 | 0.027 |
| Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)* | 552 | 578 | 90000 | 0.178 |
References
View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
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