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FISHaqua™-dUTP *1 mM in Tris Buffer (pH 7.5)*
Product key features
• Effective incorporation into DNAs
• Spectrally similar to SpectrumAqua
• An excellent replacement for SpectrumAqua
• High photostability
• Spectrally similar to SpectrumAqua
• An excellent replacement for SpectrumAqua
• High photostability
Product description
FISHaqua™-dUTP can be readily incorporated into DNAs in the presence of a polymerase. It is an excellent substrate for labeling DNAs for the Fluorescence in situ hybridization (FISH) (FISH) applications. FISHaqua™-dUTP has the same spectral properties well compatible with the filter set of SpectrumAqua™. It is used as a blue fluorescence color with SpectrumAqua™ filter set. It provides a bright signal that has high photostability and is not affected by pH. The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. SpectrumAqua™ is the trademark of Vysis.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| DEAC-dUTP *1 mM in TE Buffer (pH 7.5)* | 411 | 472 | - | 0.12 |
| Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)* | 552 | 578 | 90000 | 0.178 |
| TF1-dUTP *1 mM in TE Buffer (pH 7.5)* | 420 | 461 | 35000 | - |
| TF2-dUTP *1 mM in TE Buffer (pH 7.5)* | 503 | 525 | 75000 | 0.09 |
| TF3-dUTP *1 mM in TE Buffer (pH 7.5)* | 553 | 578 | 750001 | 0.179 |
| TF4-dUTP *1 mM in TE Buffer (pH 7.5)* | 577 | 602 | 90000 | 0.436 |
| TF5-dUTP *1 mM in TE Buffer (pH 7.5)* | 649 | 663 | 250000 | 0.027 |
References
View all 50 references: Citation Explorer
RNAscope Multiplex FISH Signal Assessment in FFPE and Fresh Frozen Tissues: The Effect of Archival Duration on RNA Expression.
Authors: Sari, Ariestya Indah Permata and Copeland, Katherine and Nuwongsri, Pattarin and Pipatsakulroj, Wiriya and Jinawath, Artit and Israsena, Nipan and Lertsittichai, Panuwat and Chirappapha, Prakasit and Shiao, Meng-Shin and Jinawath, Natini
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2025): 9-28
Authors: Sari, Ariestya Indah Permata and Copeland, Katherine and Nuwongsri, Pattarin and Pipatsakulroj, Wiriya and Jinawath, Artit and Israsena, Nipan and Lertsittichai, Panuwat and Chirappapha, Prakasit and Shiao, Meng-Shin and Jinawath, Natini
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2025): 9-28
Development of a quadruple Taqman probe-based real-time fluorescent quantitative PCR for the detection of bacterial pathogens in a marine fish.
Authors: Dong, Yuchen and Hu, Liping and Zhang, Jianbai and Zhou, Dandan and Zhang, Binzhe and Li, Xuepeng and Zhang, Jian
Journal: Microbial pathogenesis (2025): 107459
Authors: Dong, Yuchen and Hu, Liping and Zhang, Jianbai and Zhou, Dandan and Zhang, Binzhe and Li, Xuepeng and Zhang, Jian
Journal: Microbial pathogenesis (2025): 107459
SABER-FISH in Hydractinia.
Authors: Salinas-Saavedra, Miguel
Journal: Methods in molecular biology (Clifton, N.J.) (2024): 77-85
Authors: Salinas-Saavedra, Miguel
Journal: Methods in molecular biology (Clifton, N.J.) (2024): 77-85
SnapFISH-IMPUTE: an imputation method for multiplexed DNA FISH data.
Authors: Yu, Hongyu and Wu, Daiqing and Mishra, Shreya and Shen, Guning and Sun, Huaigu and Hu, Ming and Li, Yun
Journal: Communications biology (2024): 834
Authors: Yu, Hongyu and Wu, Daiqing and Mishra, Shreya and Shen, Guning and Sun, Huaigu and Hu, Ming and Li, Yun
Journal: Communications biology (2024): 834
Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP.
Authors: Perez, Monika W and Camplisson, Conor K and Beliveau, Brian J
Journal: Methods in molecular biology (Clifton, N.J.) (2024): 177-189
Authors: Perez, Monika W and Camplisson, Conor K and Beliveau, Brian J
Journal: Methods in molecular biology (Clifton, N.J.) (2024): 177-189
Page updated on April 15, 2025
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