AssayWise Letters

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SiRNA labeling and delivery into cells
Small interfering RNAs (siRNAs) are short double-stranded RNA molecules, ranging 20-25 base pairs in length. They are known to play a crucial role in gene regulation by modulating RNA interference (RNAi) pathway. The ability to control gene expression has made siRNAs a potential therapeutic target for diverse diseases. Delivering and visualizing the siRNAs, both in-vitro and in-vivo, to gain insights into their uptake and understanding the mechanism of action requires development of labeling technology that can provide detection of small amounts of siRNAs. Fluorescent labeling of siRNA enables the visualization, quantification, and tracking of siRNAs within cells and tissues. Monitoring the delivery, uptake, and distribution of siRNAs by detecting the emission from fluorescent tags provides a convenient solution. Additionally, fluorescently labeled siRNAs can also validate their targeting specificity. Real-time monitoring of gene silencing effects becomes possible, facilitating assessments of siRNA pharmacokinetics studies. The fairly simple method of labeling siRNAs and delivering them to the cells as described in this study provides an excellent solution for fundamental research on siRNAs and their therapeutic applications through RNAi pathway. Read latest issue ...