Calculator
AssayWise Letters 2013, Vol. 2(2)
In this issue
Monitoring Cell Cycles
Multicolor Labeling of Cell Nucleus with DNA-Binding Dyes
DAX-J2™ Ratio 580/460, a Ratiometric Nitric Oxide (NO) Probe
Neuraminidase Assay
Detecting Sphingomyelinase Activity
Multiplexing Caspase Activity Detection
Ratio Imaging of Cells with RatioWorks™ Probes
Multicolor Labeling of Dead Cells for Flow Cytometric Analysis
iFluor™ Labeling Probes
iFluor™ Phalloidin Conjugates for Labeling F-actins
trFluor™ Bioconjugates for Developing No Wash ELISA Assays
AssayWise Letters 2013, Vol. 2(2)
Featured Article
Monitoring Cell Cycles
There are a variety of parameters that can be used for monitoring cell viability and proliferation. In normal cells, DNA density changes depending on whether the cell is growing, dividing, resting or performing its ordinary functions. Cell Meter™ Fluorimetric Cell Cycle Assay Kits are designed to monitor cell cycle progression and proliferation by using our proprietary cell cycle dye in permeabilized and fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of the cell cycle dye is directly proportional to DNA content.…
There are a variety of parameters that can be used for monitoring cell viability and proliferation. In normal cells, DNA density changes depending on whether the cell is growing, dividing, resting or performing its ordinary functions. Cell Meter™ Fluorimetric Cell Cycle Assay Kits are designed to monitor cell cycle progression and proliferation by using our proprietary cell cycle dye in permeabilized and fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of the cell cycle dye is directly proportional to DNA content.…
Multicolor Labeling of Cell Nucleus with DNA-Binding Dyes
The cellular nucleus is central both literally and figuratively to the activities and lifecycle of the cell. As the nucleus is protected by both the cytoplasm of the cell and a double-membrane nuclear envelope, it is experimentally difficult to perform staining on cellular DNA to chart cell phase and…
The cellular nucleus is central both literally and figuratively to the activities and lifecycle of the cell. As the nucleus is protected by both the cytoplasm of the cell and a double-membrane nuclear envelope, it is experimentally difficult to perform staining on cellular DNA to chart cell phase and…
DAX-J2™ Ratio 580/460, a Ratiometric Nitric Oxide (NO) Probe
The newest addition to the DAX-J2TM Probe series is the nitric oxide (NO) sensor DAX-J2TM Ratio 580/460, which monitors NO by permeating living cells and inducing fluorescence when NO is oxidized. DAX-J2TM Ratio 580/460 improves on older probes in that it is pH-independent and requires no intracellular…
The newest addition to the DAX-J2TM Probe series is the nitric oxide (NO) sensor DAX-J2TM Ratio 580/460, which monitors NO by permeating living cells and inducing fluorescence when NO is oxidized. DAX-J2TM Ratio 580/460 improves on older probes in that it is pH-independent and requires no intracellular…
Neuraminidase Assay
Viral neuraminidase is the enzyme that facilitates the spread of influenza throughout the respiratory system by permitting the transport of the virus from infected cells through mucin. As such, this enzyme is a main target for development of influenza treatments and its presence is a hallmark of infection.…
Viral neuraminidase is the enzyme that facilitates the spread of influenza throughout the respiratory system by permitting the transport of the virus from infected cells through mucin. As such, this enzyme is a main target for development of influenza treatments and its presence is a hallmark of infection.…
Detecting Sphingomyelinase Activity
Cellular response to stress varies, but the enzyme sphingomyelinase (SMase) is linked to selected biochemical reactions and can be quantified as a useful measure of cell state. The AmpliteTM Sphingomyelinase Assay kits can be analyzed by either fluorescence or absorption readings, allowing experimental…
Cellular response to stress varies, but the enzyme sphingomyelinase (SMase) is linked to selected biochemical reactions and can be quantified as a useful measure of cell state. The AmpliteTM Sphingomyelinase Assay kits can be analyzed by either fluorescence or absorption readings, allowing experimental…
Multiplexing Caspase Activity Detection
One of the first signs of apoptosis (cell death) is the activation of caspase enzymes, which mediate multiple biochemical events in the death of the cell. Precise, sensitive measurement of the presence of these enzymes is valuable for determining the stage and rate of apoptosis. To efficiently monitor…
One of the first signs of apoptosis (cell death) is the activation of caspase enzymes, which mediate multiple biochemical events in the death of the cell. Precise, sensitive measurement of the presence of these enzymes is valuable for determining the stage and rate of apoptosis. To efficiently monitor…
Ratio Imaging of Cells with RatioWorks™ Probes
One of the first signs of apoptosis (cell death) is the activation of caspase enzymes, which mediate multiple biochemical events in the death of the cell. Precise, sensitive measurement of the presence of these enzymes is valuable for determining the stage and rate of apoptosis. To efficiently monitor…
One of the first signs of apoptosis (cell death) is the activation of caspase enzymes, which mediate multiple biochemical events in the death of the cell. Precise, sensitive measurement of the presence of these enzymes is valuable for determining the stage and rate of apoptosis. To efficiently monitor…
Multicolor Labeling of Dead Cells for Flow Cytometric Analysis
The Live or DeadTM Fixable Dead Cell Staining Kit series exploits a proprietary stain targeted to cell components that is not permeable to live cell membranes. Only when a cell surface is compromised, such as in apoptotic cells, will the stain permeate throughout the entire cell. For living cells, only…
The Live or DeadTM Fixable Dead Cell Staining Kit series exploits a proprietary stain targeted to cell components that is not permeable to live cell membranes. Only when a cell surface is compromised, such as in apoptotic cells, will the stain permeate throughout the entire cell. For living cells, only…
iFluor™ Labeling Probes
iFluor™ dyes are a series of excellent fluorescent labeling dyes that span the full UV-visible and near IR spectrum. All the iFluor™ dyes have excellent water solubility. Their hydrophilic property makes their protein conjugation readily performed in aqueous media, minimizing the use of organic…
iFluor™ dyes are a series of excellent fluorescent labeling dyes that span the full UV-visible and near IR spectrum. All the iFluor™ dyes have excellent water solubility. Their hydrophilic property makes their protein conjugation readily performed in aqueous media, minimizing the use of organic…
iFluor™ Phalloidin Conjugates for Labeling F-actins
AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging of F-actin. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual…
AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging of F-actin. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual…
trFluor™ Bioconjugates for Developing No Wash ELISA Assays
Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules…
Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules…