Multicolor Labeling of Cell Nucleus with DNA-Binding Dyes

The nucleus is the largest cellular organelle in animals. In mammalian cells, the average diameter of the nucleus is approximately 6 µm, which occupies about 10% of the total cell volume. Nucleus contains most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. The genes within these chromosomes are the cell's nuclear genome. The function of the nucleus is to maintain the integrity of these genes and to control the activities of the cell by regulating gene expression, therefore, the nucleus is the control center of the cell. The main structures making up the nucleus are the nuclear envelope, a double membrane that encloses the entire organelle and isolates its contents from the cellular cytoplasm, and the nucleoskeleton. Movement of large molecules through the pores is required for both gene expression and the maintenance of chromosomes.

Labeling the Nuclei of Live Cells

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Nuclear Green™ LCS1, Nuclear Orange™ LCS1, Nuclear Red™ LCS1 and Nuclear Yellow are fluorogenic, DNA-selective and cell-permeant dyes for analyzing DNA content in living cells. The fluorescence of these dyes is significantly enhanced upon binding to DNA. They can be used in fluorescence imaging, microplate and flow cytometry applications. These DNA-binding dyes might be used for multicolor analysis of live cells with proper filter sets.

Our recently developed Nuclear Blue™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Blue™ LCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. It is well excited by violet laser at 405 nm, and emits blue/cyan fluorescence light with an emission maximum at ~440 nm, and provides an excellent tool for flow cytometers equiped with a 405 nm violet laser source. This DNA-binding dye might be used for multicolor analysis of live cells with the filter sets of Pacific Blue™ and BD Horizon™ V450.

 

Labeling the Nuclei of Dead Cells

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Nuclear Green™ DCS1, Nuclear Orange™ DCS1 and Nuclear Red™ DCS1 are fluorogenic, DNA-selective and cell-impermeant dyes for analyzing DNA content in dead, fixed or apoptotic cells. As the LCS1 reagents, the fluorescence of the DCS1 dyes is significantly enhanced upon binding to DNA. They can be used in fluorescence imaging, microplate and flow cytometry applications. These DNA-binding dyes might be used for multicolor analysis of dead, fixed or apoptotic cells with proper filter sets.