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AssayWise Letters 2020, Vol. 9(2)
In this issue
Development and Applications of Coronavirus Protease-Specific Covidyte™ and Covipyte™ Peptide Substrates
Prolonged Retention of Calbryte™ 520 AM for In Vitro Analysis
T Lymphocyte Immunophenotyping Using Multicolor Flow Cytometry
A Practical Guide for Labeling Antibodies
AssayWise Letters 2020, Vol. 9(2)
Featured Article
Development and Applications of Coronavirus Protease-Specific Covidyte™ and Covipyte™ Peptide Substrates
There is a high level of similarity in both amino acid sequence and structure between the SARS and SARS-CoV-2 virus, particularly in regards to the catalytic active sites of their proteases. Several coronavirus-specific amino acid sequences were discovered for use in investigating enzyme inhibitors for SARS, and have since been repurposed for SARS-CoV-2 research. By conjugating these sequences to FRET pairs, several fluorescent substrates have been developed to accurately measure protease activity. These substrates can be employed in sensitive assays for research into development…
There is a high level of similarity in both amino acid sequence and structure between the SARS and SARS-CoV-2 virus, particularly in regards to the catalytic active sites of their proteases. Several coronavirus-specific amino acid sequences were discovered for use in investigating enzyme inhibitors for SARS, and have since been repurposed for SARS-CoV-2 research. By conjugating these sequences to FRET pairs, several fluorescent substrates have been developed to accurately measure protease activity. These substrates can be employed in sensitive assays for research into development…
Prolonged Retention of Calbryte™ 520 AM for In Vitro Analysis
Calcium-imaging is a sensitive method for monitoring the spatiotemporal dynamics of calcium signaling. Since intracellular concentrations are correlated with physiological and pathophysiological characteristics of the central nervous system (CNS), calcium imaging with fluorescent indicators…
Calcium-imaging is a sensitive method for monitoring the spatiotemporal dynamics of calcium signaling. Since intracellular concentrations are correlated with physiological and pathophysiological characteristics of the central nervous system (CNS), calcium imaging with fluorescent indicators…
T Lymphocyte Immunophenotyping Using Multicolor Flow Cytometry
Cluster of differentiation (CD) antigens are a complex and highly organized collection of surface molecules expressed on cells of the immune system that play key roles in immune cell-cell communication, sensing the microenvironment and in adaptive immunity. During lymphocyte maturation, immune…
Cluster of differentiation (CD) antigens are a complex and highly organized collection of surface molecules expressed on cells of the immune system that play key roles in immune cell-cell communication, sensing the microenvironment and in adaptive immunity. During lymphocyte maturation, immune…
A Practical Guide for Labeling Antibodies
Labeling antibodies with a specific, detectable tag is an essential technique for many biological research fields. The labels used range from fluorescent dyes to hapten molecules like biotin, and many more, with enzyme tags gaining popularity in recent years for use in assays. The labels are generally…
Labeling antibodies with a specific, detectable tag is an essential technique for many biological research fields. The labels used range from fluorescent dyes to hapten molecules like biotin, and many more, with enzyme tags gaining popularity in recent years for use in assays. The labels are generally…