AssayWise Letters 2023, Vol. 12
New Uses for LAMP (Loop-Mediated Isothermal Amplification)
One of the newest methods of nucleic acid amplification, loop-mediated isothermal amplification (LAMP), is a quickly-evolving alternative to PCR that is simpler to perform and has great potential to provide quick point-of-care diagnostics and screening for multiple diseases. This adaptable…
One of the newest methods of nucleic acid amplification, loop-mediated isothermal amplification (LAMP), is a quickly-evolving alternative to PCR that is simpler to perform and has great potential to provide quick point-of-care diagnostics and screening for multiple diseases. This adaptable…
Featured Article
Investigating Intercellular Channels: Focus on Gap Junctions
One of the main three types of intercellular junctions, gap junctions are crucial for several cell communication pathways, and any dysregulation can have immediate and lethal effects. They have been implicated in tumor development and the inflammatory response in general, as well as standard cellular regulation. Given this importance, being able to investigate gap junction activity is useful for several fields of study. Using fluorescent labels is one of the simpler ways to experimentally determine gap junction activity within a cell population. Fluorescent labels can be adapted and…
One of the main three types of intercellular junctions, gap junctions are crucial for several cell communication pathways, and any dysregulation can have immediate and lethal effects. They have been implicated in tumor development and the inflammatory response in general, as well as standard cellular regulation. Given this importance, being able to investigate gap junction activity is useful for several fields of study. Using fluorescent labels is one of the simpler ways to experimentally determine gap junction activity within a cell population. Fluorescent labels can be adapted and…
Unlocking the Potential of 3D Cell Culture: A Guide to Assay Optimization
The exploration of three-dimensional (3D) cell cultures in contemporary biomedical research reveals their pivotal role as a bridge between conventional two-dimensional (2D) models and the intricacies of in vivo systems. It meticulously addresses the hurdles encountered during the transition…
The exploration of three-dimensional (3D) cell cultures in contemporary biomedical research reveals their pivotal role as a bridge between conventional two-dimensional (2D) models and the intricacies of in vivo systems. It meticulously addresses the hurdles encountered during the transition…
Comprehensive Guide to Cell Health Assays in Flow Cytometry: A Multidimensional Exploration
Flow cytometry has emerged as an invaluable tool for biomedical research, enabling the nuanced assessment of cell health through a spectrum of assays. The precise evaluation of cellular vitality, viability, apoptosis, and stress responses has far-reaching implications across diverse scientific…
Flow cytometry has emerged as an invaluable tool for biomedical research, enabling the nuanced assessment of cell health through a spectrum of assays. The precise evaluation of cellular vitality, viability, apoptosis, and stress responses has far-reaching implications across diverse scientific…