Calculator
AssayWise Letters 2021, Vol. 10(1)
In this issue
Low-cost, ultra-sensitive fluorescence detection of DNA by gel electrophoresis using environmentally benign Gelite™ Safe DNA Gel Stain
Enzymatic and Chemical Labeling Strategies for Oligonucleotides
Sensitive Identification of Newly Synthesized DNA In Situ using click chemistry-based Bucculite™ XdU Cell Proliferation Assays
A Practical Guide for the Detection and Analysis of PCR Products
AssayWise Letters 2021, Vol. 10(1)
Featured Article
Low-cost, ultra-sensitive fluorescence detection of DNA by gel electrophoresis using environmentally benign Gelite™ Safe DNA Gel Stain
Gelite™ Safe DNA Gel Stain has been developed specifically to be a less hazardous alternative to ethidium bromide (EtBr) and SYBR® Green dyes for staining DNA in agarose and polyacrylamide gels, without sacrificing detection sensitivity. The exceptional sensitivity and strong DNA binding affinity of Gelite™ Safe DNA Gel Stain allows for DNA to be stained without any requirement for destaining. In addition to its superior binding properties, Gelite™ Safe DNA Gel Stain is essentially non-fluorescent in the absence of nucleic acids, displaying very low gel matrix background fluorescence.…
Gelite™ Safe DNA Gel Stain has been developed specifically to be a less hazardous alternative to ethidium bromide (EtBr) and SYBR® Green dyes for staining DNA in agarose and polyacrylamide gels, without sacrificing detection sensitivity. The exceptional sensitivity and strong DNA binding affinity of Gelite™ Safe DNA Gel Stain allows for DNA to be stained without any requirement for destaining. In addition to its superior binding properties, Gelite™ Safe DNA Gel Stain is essentially non-fluorescent in the absence of nucleic acids, displaying very low gel matrix background fluorescence.…
Enzymatic and Chemical Labeling Strategies for Oligonucleotides
Labeling strategies for oligonucleotides (oligos) differ somewhat from those for other commonly encountered biopolymers, such as proteins and carbohydrates, since oligos are exceptionally inert to common bioconjugation reagents under standard reaction conditions. While reagents are available…
Labeling strategies for oligonucleotides (oligos) differ somewhat from those for other commonly encountered biopolymers, such as proteins and carbohydrates, since oligos are exceptionally inert to common bioconjugation reagents under standard reaction conditions. While reagents are available…
Sensitive Identification of Newly Synthesized DNA In Situ using click chemistry-based Bucculite™ XdU Cell Proliferation Assays
The measurement of cell proliferation in situ is fundamental to the assessment of cell health, genotoxicity, and drug efficacy evaluation. Precisely labeling and quantifying cells in the synthesis phase (S phase) of cell cycle progression is not only adequate for characterizing basic biology but…
The measurement of cell proliferation in situ is fundamental to the assessment of cell health, genotoxicity, and drug efficacy evaluation. Precisely labeling and quantifying cells in the synthesis phase (S phase) of cell cycle progression is not only adequate for characterizing basic biology but…
A Practical Guide for the Detection and Analysis of PCR Products
PCR, or polymerase chain reaction, is a core technology used routinely to amplify a specific DNA sequence rapidly. Offering exceptional speed, specificity, and sensitivity as a tool, PCR, and its many adaptations, such as real-time PCR and reverse transcription-PCR, have revolutionized how DNA…
PCR, or polymerase chain reaction, is a core technology used routinely to amplify a specific DNA sequence rapidly. Offering exceptional speed, specificity, and sensitivity as a tool, PCR, and its many adaptations, such as real-time PCR and reverse transcription-PCR, have revolutionized how DNA…