Wheat Germ Agglutinin (WGA)

XFD350 Same Structure to Alexa Fluor® 350

XFD350, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 350, a trademark of ThermoFisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives, and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD350 WGA conjugate, like its counterpart Alexa Fluor® 350 WGA conjugate, emits a bright blue fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Wheat Germ Agglutinin, XFD488 Labeled XFD488 Same Structure to Alexa Fluor™ 488	499	520	71000	0.92¹	0.30	0.11
Wheat Germ Agglutinin, XFD594 Labeled XFD594 Same Structure to Alexa Fluor™ 594	590	618	90000	0.66¹	0.43	0.56
Wheat Germ Agglutinin, XFD532 Labeled XFD532 Same Structure to Alexa Fluor® 532	534	553	81000	0.61¹	0.24	0.09
Wheat Germ Agglutinin, XFD555 Labeled XFD555 Same Structure to Alexa Fluor® 555	553	568	150000	0.1¹	0.08	0.08
Wheat Germ Agglutinin, XFD647 Labeled XFD647 Same Structure to Alexa Fluor® 647	650	671	239000	0.33¹	0.00	0.03
Wheat Germ Agglutinin, XFD750 Labeled XFD750 Same Structure to Alexa Fluor® 750	752	776	240000	0.12¹	0.00	0.04

References

View all 50 references: Citation Explorer

Wheat Germ Agglutinin Affinity Chromatography Enrichment and Glyco-Proteomic Characterization of Tetrodotoxin-Binding Proteins from the Plasma of Cultured Tiger Pufferfish (Takifugu rubripes).
Authors: Zhang, Yafei and Minami, Ryoma and Tatsuno, Ryohei and Gao, Wei and Ueno, Mikinori and Yamada, Akinori and Yoshida, Asami and Sedanza, Mary Grace and Arima, Kazunari and Takatani, Tomohiro and Yamaguchi, Kenichi and Oshima, Yuji and Arakawa, Osamu
Journal: Bioscience, biotechnology, and biochemistry (2023)

Microscopic observation of human airway ciliary movement using wheat germ agglutinin.
Authors: Nakamura, Ryosuke and Oyagi, Seiji and Katsuno, Tatsuya and Kishimoto, Yo and Omori, Koichi
Journal: Methods in cell biology (2023): 33-43

Ultrasensitive Fluorescence Lateral Flow Assay for Simultaneous Detection of Pseudomonas aeruginosa and Salmonella typhimurium via Wheat Germ Agglutinin-Functionalized Magnetic Quantum Dot Nanoprobe.
Authors: Tu, Zhijie and Yang, Xingsheng and Dong, Hao and Yu, Qing and Zheng, Shuai and Cheng, Xiaodan and Wang, Chongwen and Rong, Zhen and Wang, Shengqi
Journal: Biosensors (2022)

Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags.
Authors: Tu, Zhijie and Cheng, Siyun and Dong, Hao and Wang, Wenqi and Yang, Xingsheng and Gu, Bing and Wang, Shengqi and Wang, Chongwen
Journal: RSC advances (2022): 27344-27354

Biogenesis of Exosomes Laden with Metallic Silver-Copper Nanoparticles Liaised by Wheat Germ Agglutinin for Targeted Delivery of Therapeutics to Breast Cancer.
Authors: Ashraf, Sarmadia and Qadri, Shahnaz and Akbar, Shayista and Parray, Aijaz and Haik, Yousef
Journal: Advanced biology (2022): e2200005

Page updated on October 8, 2024

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Physical properties

Solvent

Water

Spectral properties

Correction Factor (260 nm)	0.25
Correction Factor (280 nm)	0.19
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	343
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501