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Tris NTA succinimidyl ester
Product key features
- Tris NTA compounds have extremely high affinity to polyHIS tag proteins
- An excellent building block to develop polyHIS probes
- NHS esters readily react with amino compounds
Product key features
Tris NTA succinimidyl ester is an excellent building block to develop probes for detecting polyHIS tag proteins. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest also offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.
References
View all 33 references: Citation Explorer
Engineering Protein Nanoparticles Functionalized with an Immunodominant Coxiella burnetii Antigen to Generate a Q Fever Vaccine.
Authors: Ramirez, Aaron and Felgner, Jiin and Jain, Aarti and Jan, Sharon and Albin, Tyler J and Badten, Alexander J and Gregory, Anthony E and Nakajima, Rie and Jasinskas, Algimantas and Felgner, Philip L and Burkhardt, Amanda M and Davies, D Huw and Wang, Szu-Wen
Journal: Bioconjugate chemistry (2023): 1653-1666
Authors: Ramirez, Aaron and Felgner, Jiin and Jain, Aarti and Jan, Sharon and Albin, Tyler J and Badten, Alexander J and Gregory, Anthony E and Nakajima, Rie and Jasinskas, Algimantas and Felgner, Philip L and Burkhardt, Amanda M and Davies, D Huw and Wang, Szu-Wen
Journal: Bioconjugate chemistry (2023): 1653-1666
Protein Nanoparticle-Mediated Delivery of Recombinant Influenza Hemagglutinin Enhances Immunogenicity and Breadth of the Antibody Response.
Authors: Badten, Alexander J and Ramirez, Aaron and Hernandez-Davies, Jenny E and Albin, Tyler J and Jain, Aarti and Nakajima, Rie and Felgner, Jiin and Davies, D Huw and Wang, Szu-Wen
Journal: ACS infectious diseases (2023): 239-252
Authors: Badten, Alexander J and Ramirez, Aaron and Hernandez-Davies, Jenny E and Albin, Tyler J and Jain, Aarti and Nakajima, Rie and Felgner, Jiin and Davies, D Huw and Wang, Szu-Wen
Journal: ACS infectious diseases (2023): 239-252
A Four-Channel Surface Plasmon Resonance Sensor Functionalized Online for Simultaneous Detections of Anti-SARS-CoV-2 Antibody, Free Viral Particles, and Neutralized Viral Particles.
Authors: Dong, Tianbao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: ACS sensors (2022): 3560-3570
Authors: Dong, Tianbao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: ACS sensors (2022): 3560-3570
Regenerable and high-throughput surface plasmon resonance assay for rapid screening of anti-SARS-CoV-2 antibody in serum samples.
Authors: Jiang, Meng and Dong, Tianbao and Han, Chaowei and Liu, Luyao and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: Analytica chimica acta (2022): 339830
Authors: Jiang, Meng and Dong, Tianbao and Han, Chaowei and Liu, Luyao and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng
Journal: Analytica chimica acta (2022): 339830
Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule-Associated Intracellular Function.
Authors: Ghosh, Satyajit and Garg, Shubham and Mukherjee, Nabanita and Ghosh, Surajit
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 17-45
Authors: Ghosh, Satyajit and Garg, Shubham and Mukherjee, Nabanita and Ghosh, Surajit
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 17-45
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