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Tris NTA amine
Product key features
- Tris NTA compounds have extremely high affinity to polyHIS tag proteins
- An excellent building block to develop polyHIS probes
- Amine compounds can be readily conjugated to carbonyl compounds
Product description
Tris NTA amine is an excellent building block to develop probes for detecting polyHIS tag proteins. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.
References
View all 50 references: Citation Explorer
Development of an enzymatic aptasensor for monitoring recombinant His-tagged proteins in microbial biotechnology.
Authors: Jadidi, Mohammad Javad and Emamzadeh, Rahman and Nazari, Mahboobeh and Zaker, Sayed Rasoul
Journal: Enzyme and microbial technology (2025): 110603
Authors: Jadidi, Mohammad Javad and Emamzadeh, Rahman and Nazari, Mahboobeh and Zaker, Sayed Rasoul
Journal: Enzyme and microbial technology (2025): 110603
Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture.
Authors: Aguiar, Tatiana Q and Leal, Tânia and Rodrigues, Diana G and Abrunhosa, Luís and Oliveira, Carla and Domingues, Lucília
Journal: Talanta (2025): 127126
Authors: Aguiar, Tatiana Q and Leal, Tânia and Rodrigues, Diana G and Abrunhosa, Luís and Oliveira, Carla and Domingues, Lucília
Journal: Talanta (2025): 127126
Ni2+-induced selective precipitation of His-tagged recombinant proteins shortens purification time while maintaining high yield.
Authors: Islam, Md Din and Islam, M Monirul and Kuroda, Yutaka
Journal: Journal of biotechnology (2025): 38-46
Authors: Islam, Md Din and Islam, M Monirul and Kuroda, Yutaka
Journal: Journal of biotechnology (2025): 38-46
Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins.
Authors: Probst, Anna and Knochenhauer, Doreen and Niemeyer, Justus and Fischer, Laura and Schroda, Michael
Journal: Current genetics (2025): 7
Authors: Probst, Anna and Knochenhauer, Doreen and Niemeyer, Justus and Fischer, Laura and Schroda, Michael
Journal: Current genetics (2025): 7
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character.
Authors: Yang, Huizhen and Zhao, Ziyan and Li, Hongquan and Wang, Lan
Journal: Protein expression and purification (2024): 106519
Authors: Yang, Huizhen and Zhao, Ziyan and Li, Hongquan and Wang, Lan
Journal: Protein expression and purification (2024): 106519
Page updated on April 15, 2025
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