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Tris NTA maleimide
Product key features
- Tris NTA compounds have extremely high affinity to polyHIS tag proteins
- An excellent building block to develop polyHIS probes
- Maleimides readily react with thiol compounds
Product description
Tris NTA malemide is an excellent building block to develop probes for detecting polyHIS tag proteins. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.
References
View all 9 references: Citation Explorer
Expression and purification of recombinant arginine decarboxylase (speA) from Escherichia coli.
Authors: Song, Jiaping and Zhou, Chuanwen and Liu, Rui and Wu, Xudong and Wu, Di and Hu, Xiaojian and Ding, Yu
Journal: Molecular biology reports (2010): 1823-9
Authors: Song, Jiaping and Zhou, Chuanwen and Liu, Rui and Wu, Xudong and Wu, Di and Hu, Xiaojian and Ding, Yu
Journal: Molecular biology reports (2010): 1823-9
[Isolation, prokaryotic expression and activity analysis of thymidylate kinase (tmk) gene from Phytoplasma of wheat blue dwarf].
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44
Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification.
Authors: Jonák, J
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2007): 141-53
Authors: Jonák, J
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2007): 141-53
[Displaying polyhistidine peptide on the cell surface of Bacillus thuringiensis by S-layer protein].
Authors: Wang, Li and Sun, Ming and Yu, Zi Niu
Journal: Shi yan sheng wu xue bao (2003): 476-81
Authors: Wang, Li and Sun, Ming and Yu, Zi Niu
Journal: Shi yan sheng wu xue bao (2003): 476-81
Lack of antitumor activity of recombinant endostatin in a human neuroblastoma xenograft model.
Authors: Jouanneau, E and Alberti, L and Nejjari, M and Treilleux, I and Vilgrain, I and Duc, A and Combaret, V and Favrot, M and Leboulch, P and Bachelot, T
Journal: Journal of neuro-oncology (2001): 11-8
Authors: Jouanneau, E and Alberti, L and Nejjari, M and Treilleux, I and Vilgrain, I and Duc, A and Combaret, V and Favrot, M and Leboulch, P and Bachelot, T
Journal: Journal of neuro-oncology (2001): 11-8
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