Calculator
Tris NTA azide
Product key features
- Tris NTA compounds have extremely high affinity to polyHIS tag proteins
- An excellent building block to develop polyHIS probes
- Azido compounds selectively react with alkyne compounds such as DBCO
Product description
Tris NTA azide is an excellent building block to develop probes for detecting polyHIS tag proteins via the well-known click chemistry. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.
References
View all 50 references: Citation Explorer
Heterogeneous biocatalysis by magnetic nanoparticle immobilized biomass-degrading enzymes derived from microbial cultures.
Authors: Gupta, Mani and Bandyopadhyay, Arghya and Sinha, Sushant Kumar and Suksham, and Samanta, Abhisek and Mondal, Surajit and Bhattacharyya, Sayan and Datta, Supratim
Journal: Journal of materials chemistry. B (2025): 3644-3652
Authors: Gupta, Mani and Bandyopadhyay, Arghya and Sinha, Sushant Kumar and Suksham, and Samanta, Abhisek and Mondal, Surajit and Bhattacharyya, Sayan and Datta, Supratim
Journal: Journal of materials chemistry. B (2025): 3644-3652
Fabrication of lignin-based sub-micro hybrid particle as a novel support for adenylate cyclase immobilization.
Authors: Jiang, Dahai and Xu, Ting and Xiao, Honggang and Xu, Wangbo and Zhu, Qingqing and Liu, Dong and Chen, Yong and Ying, Hanjie and Niu, Huanqing and Li, Ming
Journal: Colloids and surfaces. B, Biointerfaces (2024): 113658
Authors: Jiang, Dahai and Xu, Ting and Xiao, Honggang and Xu, Wangbo and Zhu, Qingqing and Liu, Dong and Chen, Yong and Ying, Hanjie and Niu, Huanqing and Li, Ming
Journal: Colloids and surfaces. B, Biointerfaces (2024): 113658
The chromatin remodeler SMARCA5 binds to d-block metal supports: Characterization of affinities by IMAC chromatography and QM analysis.
Authors: Andrikopoulos, Prokopis C and Čabart, Pavel
Journal: PloS one (2024): e0309134
Authors: Andrikopoulos, Prokopis C and Čabart, Pavel
Journal: PloS one (2024): e0309134
Simultaneous Identification of Vitamins B1, B3, B5, and B6 by an Engineered Nanopore.
Authors: Ouyang, Yusheng and Wang, Kefan and Jia, Wendong and Zhang, Panke and Huang, Shuo
Journal: Nano letters (2024): 11944-11953
Authors: Ouyang, Yusheng and Wang, Kefan and Jia, Wendong and Zhang, Panke and Huang, Shuo
Journal: Nano letters (2024): 11944-11953
Histidine tag modified magnetic beads for analysis of arsenic binding proteins.
Authors: Nan, Kai and He, Man and Chen, Beibei and Hu, Bin
Journal: Analytica chimica acta (2024): 342554
Authors: Nan, Kai and He, Man and Chen, Beibei and Hu, Bin
Journal: Analytica chimica acta (2024): 342554
Page updated on April 15, 2025
Safety data sheet