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Tris NTA alkyne
Product key features
- Tris NTA compounds have extremely high affinity to polyHIS tag proteins
- An excellent building block to develop polyHIS probes
- Alkyne compounds selectively react with azido compounds
Product description
Tris NTA alkyne is an excellent building block to develop probes for detecting polyHIS tag proteins via the well-known click chemistry. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.
References
View all 50 references: Citation Explorer
Differential structural characteristics, physicochemical properties, and calcium-binding capabilities of annexin A2 wild-type versus E53A, E96A, D162A, E247A and D322A mutants.
Authors: Yoodee, Sunisa and Plumworasawat, Sirikanya and Malaitad, Thanyalak and Peerapen, Paleerath and Thongboonkerd, Visith
Journal: Archives of biochemistry and biophysics (2025): 110267
Authors: Yoodee, Sunisa and Plumworasawat, Sirikanya and Malaitad, Thanyalak and Peerapen, Paleerath and Thongboonkerd, Visith
Journal: Archives of biochemistry and biophysics (2025): 110267
Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins.
Authors: Probst, Anna and Knochenhauer, Doreen and Niemeyer, Justus and Fischer, Laura and Schroda, Michael
Journal: Current genetics (2025): 7
Authors: Probst, Anna and Knochenhauer, Doreen and Niemeyer, Justus and Fischer, Laura and Schroda, Michael
Journal: Current genetics (2025): 7
Efficient periplasmic expression of active lysyl endopeptidase and optimizing the purification methods.
Authors: Pourani, Zahra and Keramati, Malihe and Komijani, Samira and Golkar, Majid and Cohan, Reza Ahangari and Mohseni, Nastaran and Valizadeh, Vahideh
Journal: Protein expression and purification (2025): 106618
Authors: Pourani, Zahra and Keramati, Malihe and Komijani, Samira and Golkar, Majid and Cohan, Reza Ahangari and Mohseni, Nastaran and Valizadeh, Vahideh
Journal: Protein expression and purification (2025): 106618
Shotgun and targeted proteomics of Mycolicibacterium smegmatis highlight the role of arginine phosphorylation in the functional adaptation to its environment.
Authors: Xu, Danyang and Shi, Jiahui and Jiang, Songhao and Meng, Shuhong and Cheng, Zhiyuan and Wu, Wenhui and Chang, Lei and Xie, Yuping and Gao, Yuan and Xue, Yu and Zhang, Yao
Journal: Journal of proteomics (2025): 105388
Authors: Xu, Danyang and Shi, Jiahui and Jiang, Songhao and Meng, Shuhong and Cheng, Zhiyuan and Wu, Wenhui and Chang, Lei and Xie, Yuping and Gao, Yuan and Xue, Yu and Zhang, Yao
Journal: Journal of proteomics (2025): 105388
Heterologous expression and enzymological characterization of L-glutamate oxidase from the marine actinomycete Streptomyces lydicamycinicus NBRC 110027.
Authors: Oikawa, Tadao and Yamanaka, Kazuya
Journal: Bioscience, biotechnology, and biochemistry (2025): 371-381
Authors: Oikawa, Tadao and Yamanaka, Kazuya
Journal: Bioscience, biotechnology, and biochemistry (2025): 371-381
Page updated on April 15, 2025
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