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Thiazole orange *CAS 107091-89-4*
Thiazole orange is a fluorescent DNA-binding dye commonly used to analyze reticulocytes and Plasmodium species. Its derivatives—SYBR Green, PicoGreen, TOTO, and YOYO—are widely used for detecting DNA samples, enhancing sensitivity and accuracy in applications like PCR assays and gel electrophoresis.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Thiazole orange *CAS 107091-89-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 209.815 µL | 1.049 mL | 2.098 mL | 10.491 mL | 20.982 mL |
| 5 mM | 41.963 µL | 209.815 µL | 419.63 µL | 2.098 mL | 4.196 mL |
| 10 mM | 20.982 µL | 104.908 µL | 209.815 µL | 1.049 mL | 2.098 mL |
Molarity calculator
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Acridine orange | 490 | 520 | 27000 |
| Acridine orange *10 mg/mL solution in water* | 490 | 520 | 27000 |
| CytoTell™ Orange | 541 | 560 | - |
| LysoBrite™ Orange | 543 | 565 | - |
References
View all 52 references: Citation Explorer
Large dynamic Stokes shift of DNA intercalation dye Thiazole Orange has contribution from a high-frequency mode
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954
Effect of UVC-Induced Photooxidation of DNA on the Intercalation of Thiazole Orange: A Convenient Reporter for Oxidative Damage
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)
Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213
Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555
Exploring Base-Pair-Specific Optical Properties of the DNA Stain Thiazole Orange
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)
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