Acridine orange
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 301.82 |
Solvent | Water |
Spectral properties
Absorbance (nm) | 487 |
Extinction coefficient (cm -1 M -1) | 27000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Alternative formats
Acridine orange *10 mg/mL solution in water* |
Overview | SDSProtocol |
CAS 65-61-2 | Molecular weight 301.82 | Absorbance (nm) 487 | Extinction coefficient (cm -1 M -1) 27000 | Excitation (nm) 490 | Emission (nm) 520 |
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Acridine orange to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 331.323 µL | 1.657 mL | 3.313 mL | 16.566 mL | 33.132 mL |
5 mM | 66.265 µL | 331.323 µL | 662.647 µL | 3.313 mL | 6.626 mL |
10 mM | 33.132 µL | 165.662 µL | 331.323 µL | 1.657 mL | 3.313 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 487 |
Extinction coefficient (cm -1 M -1) | 27000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Product Family
Name | Excitation (nm) | Emission (nm) |
Thiazole orange *CAS 107091-89-4* | 514 | 533 |
Thiazole orange *10 mM in DMSO* | 514 | 533 |
CytoTell™ Orange | 541 | 560 |
LysoBrite™ Orange | 543 | 565 |
References
View all 56 references: Citation Explorer
Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Re: The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. (Mutagenesis, 20, 411-415, 2005)
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267
Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186
Vesicle disruption, plasma membrane bleb formation, and acute cell death caused by illumination with blue light in acridine orange-loaded malignant melanoma cells
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)
The use of acridine orange base (AOB) as molecular probe to characterize nonaqueous AOT reverse micelles
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356
Acridine Orange based platinum(II) complexes inducing cytotoxicity and cell cycle perturbation in spite of GSTP1 up-regulation
Authors: Valentini A, Pucci D, Crispini A, Federici G, Bernardini S.
Journal: Chem Biol Interact (2006): 241
Authors: Valentini A, Pucci D, Crispini A, Federici G, Bernardini S.
Journal: Chem Biol Interact (2006): 241
Detection of catheter-related bloodstream infections by the Gram stain-acridine orange leukocyte cytospin test in hematopoietic stem cell transplant recipients
Authors: Abdelkefi A, Achour W, Torjman L, Ben Othman T, Ladeb S, Lakhal A, Allouche H, Ben Hassen A, Ben Abdeladhim A.
Journal: Bone Marrow Transplant (2006): 595
Authors: Abdelkefi A, Achour W, Torjman L, Ben Othman T, Ladeb S, Lakhal A, Allouche H, Ben Hassen A, Ben Abdeladhim A.
Journal: Bone Marrow Transplant (2006): 595
Mutagenic effect of acridine orange on the expression of penicillin G acylase and beta-lactamase in Escherichia coli
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94
Study on the formation and depolymerization of acridine orange dimer in acridine orange-sodium dodecyl benzene sulfonate-protein system
Authors: Wang F, Yang J, Wu X, Wang X, Feng L, Jia Z, Guo C.
Journal: J Colloid Interface Sci (2006): 757
Authors: Wang F, Yang J, Wu X, Wang X, Feng L, Jia Z, Guo C.
Journal: J Colloid Interface Sci (2006): 757
Postoperative changes of sperm chromatin heterogeneity, using acridine orange staining, in varicocele patients
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223