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Acridine Orange
10 mg/mL solution in water
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Thiazole orange *CAS 107091-89-4* | 514 | 533 |
| Thiazole orange *10 mM in DMSO* | 514 | 533 |
| CytoTell™ Orange | 541 | 560 |
| LysoBrite™ Orange | 543 | 565 |
Citations
View all 3 citations: Citation Explorer
Ionizing radiation induces neurotoxicity in Xenopus laevis embryos through neuroactive ligand-receptor interaction pathway
Authors: Hou, Yue and Shang, Yue and Xu, Feifei and Li, Tingyang and Li, Min and Wei, Ling and Fan, Saijun and Hou, Wenbin and Gou, Wenfeng and Shang, Haihua and others,
Journal: Environmental Research (2024): 119237
Authors: Hou, Yue and Shang, Yue and Xu, Feifei and Li, Tingyang and Li, Min and Wei, Ling and Fan, Saijun and Hou, Wenbin and Gou, Wenfeng and Shang, Haihua and others,
Journal: Environmental Research (2024): 119237
Prevention of tumor progression in inflammation-related carcinogenesis by anti-inflammatory and anti-mutagenic effects brought about by ingesting fermented brown rice and rice bran with Aspergillus oryzae (FBRA)
Authors: Nemoto, Hideyuki and Otake, Marina and Matsumoto, Takahiro and Izutsu, Runa and Jehung, Jumond P and Goto, Keisuke and Osaki, Mitsuhiko and Mayama, Masafumi and Shikanai, Masataka and Kobayashi, Hiroshi and others,
Journal: Journal of Functional Foods (2022): 104907
Authors: Nemoto, Hideyuki and Otake, Marina and Matsumoto, Takahiro and Izutsu, Runa and Jehung, Jumond P and Goto, Keisuke and Osaki, Mitsuhiko and Mayama, Masafumi and Shikanai, Masataka and Kobayashi, Hiroshi and others,
Journal: Journal of Functional Foods (2022): 104907
Label-free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion
Authors: Zhao, Lili and Zhao, Meng and Yang, Yu and Gu, Yajun and Zheng, Fang and Wang, Xuan and Zheng, Zhiyuan and Sun, Xuguo
Journal: Oncology Letters
Authors: Zhao, Lili and Zhao, Meng and Yang, Yu and Gu, Yajun and Zheng, Fang and Wang, Xuan and Zheng, Zhiyuan and Sun, Xuguo
Journal: Oncology Letters
References
View all 56 references: Citation Explorer
The use of acridine orange base (AOB) as molecular probe to characterize nonaqueous AOT reverse micelles
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356
Re: The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. (Mutagenesis, 20, 411-415, 2005)
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267
Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186
Vesicle disruption, plasma membrane bleb formation, and acute cell death caused by illumination with blue light in acridine orange-loaded malignant melanoma cells
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)
Acridine Orange based platinum(II) complexes inducing cytotoxicity and cell cycle perturbation in spite of GSTP1 up-regulation
Authors: Valentini A, Pucci D, Crispini A, Federici G, Bernardini S.
Journal: Chem Biol Interact (2006): 241
Authors: Valentini A, Pucci D, Crispini A, Federici G, Bernardini S.
Journal: Chem Biol Interact (2006): 241
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