Thiazole orange *10 mM in DMSO*
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Physical properties
Molecular weight | 476.61 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 514 |
Emission (nm) | 533 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Alternative formats
Thiazole orange *CAS 107091-89-4* |
Overview | SDSProtocol |
See also: Nucleus
CAS 107091-89-4 | Molecular weight 476.61 | Excitation (nm) 514 | Emission (nm) 533 |
Thiazole orange is a fluorescent DNA-binding dye for reticulocyte analysis. It is lso useful for Plasmodium species analysis. The modified thiazole orange derivatives (such as SYBR Green, PicoGreen, TOTO and YOYO) are widely used for detecting DNA samples.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Thiazole orange *10 mM in DMSO* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 209.815 µL | 1.049 mL | 2.098 mL | 10.491 mL | 20.982 mL |
5 mM | 41.963 µL | 209.815 µL | 419.63 µL | 2.098 mL | 4.196 mL |
10 mM | 20.982 µL | 104.908 µL | 209.815 µL | 1.049 mL | 2.098 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Acridine orange | 490 | 520 | 27000 |
Acridine orange *10 mg/mL solution in water* | 490 | 520 | 27000 |
CytoTell™ Orange | 541 | 560 | - |
LysoBrite™ Orange | 543 | 565 | - |
References
View all 52 references: Citation Explorer
Large dynamic Stokes shift of DNA intercalation dye Thiazole Orange has contribution from a high-frequency mode
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954
Effect of UVC-Induced Photooxidation of DNA on the Intercalation of Thiazole Orange: A Convenient Reporter for Oxidative Damage
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)
Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213
Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555
Exploring Base-Pair-Specific Optical Properties of the DNA Stain Thiazole Orange
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)
Sequence-specific fluorescence detection of DNA by polyamide-thiazole orange conjugates
Authors: Fechter EJ, Olenyuk B, Dervan PB.
Journal: J Am Chem Soc (2005): 16685
Authors: Fechter EJ, Olenyuk B, Dervan PB.
Journal: J Am Chem Soc (2005): 16685
Synthesis and fluorescence studies of thiazole orange tethered onto oligonucleotide: development of a self-contained DNA biosensor on a fiber optic surface
Authors: Wang X, Krull UJ.
Journal: Bioorg Med Chem Lett (2005): 1725
Authors: Wang X, Krull UJ.
Journal: Bioorg Med Chem Lett (2005): 1725
Forced intercalation probes (FIT Probes): thiazole orange as a fluorescent base in peptide nucleic acids for homogeneous single-nucleotide-polymorphism detection
Authors: Kohler O, Jarikote DV, Seitz O.
Journal: Chembiochem (2005): 69
Authors: Kohler O, Jarikote DV, Seitz O.
Journal: Chembiochem (2005): 69
Thiazole orange positive platelets in a dog with Evans' syndrome
Authors: Michimoto T, Okamura T, Suzuki K, Watari T, Kano R, Hasegawa A.
Journal: J Vet Med Sci (2004): 1305
Authors: Michimoto T, Okamura T, Suzuki K, Watari T, Kano R, Hasegawa A.
Journal: J Vet Med Sci (2004): 1305
Thiazole orange-peptide conjugates: sensitivity of DNA binding to chemical structure
Authors: Carreon JR, Mahon KP, Jr., Kelley SO.
Journal: Org Lett (2004): 517
Authors: Carreon JR, Mahon KP, Jr., Kelley SO.
Journal: Org Lett (2004): 517