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Nuclear Violet™ DCS1 *5 mM DMSO Solution*
Our Nuclear Violet™ DCS1 is a fluorogenic, DNA-selective and live cell-impermeant dye for analyzing DNA content in dead or fixed cells. The Nuclear Violet™ DCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. It is well excited by violet laser at 405 nm, and emits blue/cyan fluorescence light around an emission maximum at ~440 nm, and provides an excellent tool for flow cytometers equipped with a 405 nm violet laser source. This DNA-binding dye might be used for multicolor analysis of live/dead cells with the filter sets of Pacific Blue and BD Horizon V450.
Example protocol
PREPARATION OF WORKING SOLUTION
Nuclear Violet™ DCS1 working solution
Dilute the Nuclear Violet™ DCS1 stock solution (5 mM) to 0.5 to 5 µM final concentration in the buffer of your choice.SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.
- Add Nuclear Violet™ DCS1 working solution into the fixed, dead or apoptotic cells (either suspension or adherent) and stain the cells for 15 to 60 minutes. In initial experiments, it may be best to try several dye concentrations to determine the optimal concentration that yields the desired result.
- Directly analyze the cellular staining with fluorescence microscopy, fluorescence microplate reader, or flow cytometry.
Note Optional: Cells can be washed with PBS prior to analysis.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Nuclear Violet™ LCS1 *5 mM DMSO Solution* | 401 | 460 |
| Nuclear Blue™ DCS1 *5 mM DMSO Solution* | 348 | 469 |
| Nuclear Green™ DCS1 *5 mM DMSO Solution* | 503 | 527 |
| Nuclear Orange™ DCS1 *5 mM DMSO Solution* | 514 | 556 |
| Nuclear Red™ DCS1 *5 mM DMSO Solution* | 642 | 657 |
Citations
View all 1 citations: Citation Explorer
Structural and molecular properties of mumps virus inclusion bodies
Authors: Katoh, Hiroshi and Kimura, Ryuichi and Sekizuka, Tsuyoshi and Matsuoka, Kohei and Hosogi, Mika and Kitai, Yuki and Akahori, Yukiko and Kato, Fumihiro and Kataoka, Michiyo and Kobayashi, Hirotaka and others,
Journal: Science Advances (2024): eadr0359
Authors: Katoh, Hiroshi and Kimura, Ryuichi and Sekizuka, Tsuyoshi and Matsuoka, Kohei and Hosogi, Mika and Kitai, Yuki and Akahori, Yukiko and Kato, Fumihiro and Kataoka, Michiyo and Kobayashi, Hirotaka and others,
Journal: Science Advances (2024): eadr0359
References
View all 50 references: Citation Explorer
Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin.
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Cordova, Miguel and Kose, Kivanc and Aleissa, Saud and Alessi-Fox, Christi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of biophotonics (2021): e202000207
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Cordova, Miguel and Kose, Kivanc and Aleissa, Saud and Alessi-Fox, Christi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of biophotonics (2021): e202000207
Assessment of laser-induced thermal damage in fresh skin with ex vivo confocal microscopy.
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of the American Academy of Dermatology (2021): e19-e21
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of the American Academy of Dermatology (2021): e19-e21
Development of a novel flow cytometry-based approach for reticulocytes micronucleus test in rat peripheral blood.
Authors: Chen, Yiyi and Huo, Jiao and Liu, Yunjie and Zeng, Zhu and Zhu, Xuejiao and Chen, Xuxi and Wu, Rui and Zhang, Lishi and Chen, Jinyao
Journal: Journal of applied toxicology : JAT (2021): 595-606
Authors: Chen, Yiyi and Huo, Jiao and Liu, Yunjie and Zeng, Zhu and Zhu, Xuejiao and Chen, Xuxi and Wu, Rui and Zhang, Lishi and Chen, Jinyao
Journal: Journal of applied toxicology : JAT (2021): 595-606
A novel method to purify neutrophils enables functional analysis of zebrafish hematopoiesis.
Authors: Konno, Katsuhiro and Kulkeaw, Kasem and Sasada, Manabu and Nii, Takenobu and Kaneyuki, Ayako and Ishitani, Tohru and Arai, Fumio and Sugiyama, Daisuke
Journal: Genes to cells : devoted to molecular & cellular mechanisms (2020): 770-781
Authors: Konno, Katsuhiro and Kulkeaw, Kasem and Sasada, Manabu and Nii, Takenobu and Kaneyuki, Ayako and Ishitani, Tohru and Arai, Fumio and Sugiyama, Daisuke
Journal: Genes to cells : devoted to molecular & cellular mechanisms (2020): 770-781
[The Establishment of a Three-color Flow Cytometry Approach for the Scoring of Micronucleated Reticulocytes in Rat Bone Marrow].
Authors: Zeng, Zhu and Zhu, Xue-Jiao and Huo, Jiao and Liu, Yun-Jie and Peng, Zi-Hao and Chen, Jin-Yao and Zhang, Li-Shi
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2020): 67-73
Authors: Zeng, Zhu and Zhu, Xue-Jiao and Huo, Jiao and Liu, Yun-Jie and Peng, Zi-Hao and Chen, Jin-Yao and Zhang, Li-Shi
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2020): 67-73
Page updated on December 17, 2024
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