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Nuclear Violet™ LCS1 *5 mM DMSO Solution*

Our Nuclear Violet™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Violet™ LCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. It is well excited by violet laser at 405 nm, and emits blue/cyan fluorescence light around an emission maximum at ~460 nm, and provides an excellent tool for flow cytometers equipped with a 405 nm violet laser source. This DNA-binding dye might be used for multicolor analysis of live cells with the filter sets of Pacific Blue and BD Horizon V450.

Spectrum

Citations

View all 10 citations: Citation Explorer
Structural and molecular properties of mumps virus inclusion bodies
Authors: Katoh, Hiroshi and Kimura, Ryuichi and Sekizuka, Tsuyoshi and Matsuoka, Kohei and Hosogi, Mika and Kitai, Yuki and Akahori, Yukiko and Kato, Fumihiro and Kataoka, Michiyo and Kobayashi, Hirotaka and others,
Journal: Science Advances (2024): eadr0359
The Molecular Signature of Neuropathic Pain in a Human Model System
Authors: Sandy-Hindmarch, Oliver and Chang, Pao-Sheng and Scheuren, Paulina Simonne and De Schoenmacker, Iara and Hubli, Michele and Loizou, Constantinou and Wirth, Stephan and Mahadevan, Devendra and Wiberg, Akira and Furniss, Dominic and others,
Journal: medRxiv (2024): 2024--01
Activation of invasion by oncogenic reprogramming of cholesterol metabolism via increased NPC1 expression and macropinocytosis
Authors: Skorda, Aikaterini and Lauridsen, Anna R{\o}ssberg and Wu, Chengnan and Huang, Jinrong and Mrackova, Monika and Winther, Nuggi Ingholt and Jank, Vanessa and Sztupinszki, Zsofia and Strauss, Robert and Bilgin, Mesut and others,
Journal: Oncogene (2023): 1--12
Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections
Authors: Honda, Mizuki and Kimura, Ryuichi and Harada, Akihito and Maehara, Kazumitsu and Tanaka, Kaori and Ohkawa, Yasuyuki and Oki, Shinya
Journal: STAR protocols (2022): 101346

References

View all 65 references: Citation Explorer
In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751
Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706
Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555
Page updated on December 17, 2024

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Catalog Number17543
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Physical properties

Molecular weight

N/A

Solvent

DMSO

Spectral properties

Excitation (nm)

401

Emission (nm)

460

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Fluorescence image of live HeLa cells stained with Nuclear Violet&trade; LCS1(Cat. 17543) and MitoLite&trade; Deep Red FX660&nbsp;(Cat. 22678) using fluorescence microscope with a DAPI and Cy5 filter set.
Fluorescence image of live HeLa cells stained with Nuclear Violet&trade; LCS1(Cat. 17543) and MitoLite&trade; Deep Red FX660&nbsp;(Cat. 22678) using fluorescence microscope with a DAPI and Cy5 filter set.
Fluorescence image of live HeLa cells stained with Nuclear Violet&trade; LCS1(Cat. 17543) and MitoLite&trade; Deep Red FX660&nbsp;(Cat. 22678) using fluorescence microscope with a DAPI and Cy5 filter set.