Nuclear Green™ Fixable DCS1 *5 mM DMSO Solution*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | DMSO |
Excitation (nm) | 510 |
Emission (nm) | 532 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | ![]() ![]() |
Excitation (nm) 510 | Emission (nm) 532 |
Platform
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Ex/Em = 510/532 nm (bound to DNA)
SAMPLE EXPERIMENTAL PROTOCOL
Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.
Add 2 to 10 µM of Nuclear Green™ DCS1 to fixed, dead, or apoptotic cells (whether in suspension or adherent) and incubate for 15 to 60 minutes.
Note: In order to determine the optimal concentration that yields the desired result, it is advisable to test a wide range of dye concentrations in initial experiments.
Optional: Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.
Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set.
Optional: Fix cells with 4% formaldehyde for 20 minutes at room temperature. Wash cells twice to get rid of any fixation solution
Images
References
Authors: Wang, Xiaoyan and Qiao, Yinuo and Zhang, Jinyang and Song, Yuzhu and Han, Qinqin
Journal: Analytical and bioanalytical chemistry (2024): 299-311
Authors: Lu, Si-Jia and Ma, Meng-Yao and Yan, Xiao-Guang and Zhao, Fu-Jie and Hu, Wen-Yang and Ding, Qing-Wen and Ren, Hao-Jie and Xiang, Yu-Qiang and Zheng, Lan-Lan
Journal: Veterinarni medicina (2023): 106-115
Authors: Jin, Yong Ju and Lee, Seung Yeup and Kong, Hyun Gi and Yang, Su In and Ham, Hyeonheui and Lee, Mi-Hyun and Park, Dong Suk
Journal: Plant disease (2023): 624-627
Authors: Hawadak, Joseph and Chaudhry, Shewta and Pande, Veena and Singh, Vineeta
Journal: Journal of pharmacological and toxicological methods (2023): 107472
Authors: Lai, Meng Yee and Ooi, Choo Huck and Lau, Yee Ling
Journal: Malaria journal (2023): 314
Authors: Spanamberg, Andréia and Ravazzolo, Ana Paula and Araujo, Ricardo and Tomazi, Natália and Fuentes, Beatriz and Ferreiro, Laerte
Journal: Medical mycology (2023)
Authors: Chang, Jiang and Wang, Nan and Zhan, Jun-Peng and Zhang, Shi-Jun and Zou, De-Ying and Li, Feng and Zhang, Ying and Li, Yan-Song and Hu, Pan and Lu, Shi-Ying and Liu, Zeng-Shan and Ren, Hong-Lin
Journal: Folia microbiologica (2023)
Authors: Lai, Meng Yee and Lau, Yee Ling
Journal: Acta tropica (2023): 107029
Authors: Hou, Yucui and Lu, Xiaoling and Yang, Jie and Tang, Chunhua and Jiang, Hanbing and Cai, Tongji and Chen, Meilun and Wei, Zheng and Yu, Peng
Journal: Analytical methods : advancing methods and applications (2023): 1546-1552
Authors: Soni, Aishwarya and Dahiya, Bhawna and Sheoran, Abhishek and Kumar, Vipul and Guliani, Astha and Kumar, Nitin and Hooda, Vikas and Yadav, Aparna and Nehra, Kiran and Mehta, Promod K
Journal: Expert review of respiratory medicine (2023): 1079-1089