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Acridine orange

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Physical properties
Molecular weight301.82
SolventWater
Spectral properties
Absorbance (nm)487
Extinction coefficient (cm -1 M -1)27000
Excitation (nm)490
Emission (nm)520
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
65-61-2
Molecular weight
301.82
Absorbance (nm)
487
Extinction coefficient (cm -1 M -1)
27000
Excitation (nm)
490
Emission (nm)
520
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Acridine orange to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM331.323 µL1.657 mL3.313 mL16.566 mL33.132 mL
5 mM66.265 µL331.323 µL662.647 µL3.313 mL6.626 mL
10 mM33.132 µL165.662 µL331.323 µL1.657 mL3.313 mL

Molarity calculator

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Spectrum


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Spectral properties

Absorbance (nm)487
Extinction coefficient (cm -1 M -1)27000
Excitation (nm)490
Emission (nm)520

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References


View all 56 references: Citation Explorer
Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Re: The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. (Mutagenesis, 20, 411-415, 2005)
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267
Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186
Vesicle disruption, plasma membrane bleb formation, and acute cell death caused by illumination with blue light in acridine orange-loaded malignant melanoma cells
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)
The use of acridine orange base (AOB) as molecular probe to characterize nonaqueous AOT reverse micelles
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356
Acridine Orange based platinum(II) complexes inducing cytotoxicity and cell cycle perturbation in spite of GSTP1 up-regulation
Authors: Valentini A, Pucci D, Crispini A, Federici G, Bernardini S.
Journal: Chem Biol Interact (2006): 241
Detection of catheter-related bloodstream infections by the Gram stain-acridine orange leukocyte cytospin test in hematopoietic stem cell transplant recipients
Authors: Abdelkefi A, Achour W, Torjman L, Ben Othman T, Ladeb S, Lakhal A, Allouche H, Ben Hassen A, Ben Abdeladhim A.
Journal: Bone Marrow Transplant (2006): 595
Mutagenic effect of acridine orange on the expression of penicillin G acylase and beta-lactamase in Escherichia coli
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94
Study on the formation and depolymerization of acridine orange dimer in acridine orange-sodium dodecyl benzene sulfonate-protein system
Authors: Wang F, Yang J, Wu X, Wang X, Feng L, Jia Z, Guo C.
Journal: J Colloid Interface Sci (2006): 757
Postoperative changes of sperm chromatin heterogeneity, using acridine orange staining, in varicocele patients
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223