XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*

• Ex/Em: 752/776 nm
• Extinction coefficient: 240,000 cm^-1M^-1
• Reactive Group: NHS ester
• Easy Conjugation: Efficiently labels primary amines on proteins, ligands, and amine-modified oligonucleotides
• Bright & Stable: Offers intense, photostable fluorescence for labeling low-abundance targets
• Water Soluble: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

XFD750, manufactured by AAT Bioquest, is a bright near-infrared fluorescent dye structurally identical to Alexa Fluor™ 750 (ThermoFisher). It is efficiently excited by the 633 nm laser line and is compatible with the Cy7 filter set, making it well-suited for applications such as fluorescence microscopy and flow cytometry. The dye demonstrates excellent aqueous solubility and maintains pH stability across a broad range (pH 4–10), ensuring reliable and reproducible fluorescence signals under diverse experimental conditions. Its long-wavelength emission effectively reduces background autofluorescence, enhancing signal-to-noise ratios in complex biological samples, particularly in tissue imaging. Furthermore, XFD750 is widely utilized in stochastic optical reconstruction microscopy (STORM), providing exceptional performance in both dSTORM and nSTORM super-resolution imaging techniques.

The N-hydroxysuccinimidyl (NHS) ester of XFD750 is a widely used reagent for the conjugation of this dye to proteins or antibodies. NHS esters react selectively and efficiently with primary amines (such as the side chains of lysine residues or aminosilane-coated surfaces) at pH 7-9, forming stable covalent amide bonds. This property makes XFD750 NHS ester an excellent choice for labeling proteins, amine-modified oligonucleotides, and other amine-containing molecules.