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XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1079.39 |
| Solvent | DMSO |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 132000 |
| Excitation (nm) | 663 |
| Emission (nm) | 691 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
Molecular weight 1079.39 | Extinction coefficient (cm -1 M -1) 132000 | Excitation (nm) 663 | Emission (nm) 691 |
XFD660, synthesized by AAT Bioquest, shares the exact same chemical structure with Alexa Fluor® 660 (a ThermoFisher trademark). This far-red dye is optimized for excitation by the 633 or 647 nm laser lines, making it well-suited for applications in imaging and flow cytometry where bright and photostable signal generation is critical. XFD660 is water-soluble and demonstrates pH stability within a range from 4 to 10. The NHS ester derivative of XFD660 is predominantly employed for conjugation to proteins or antibodies. This functionality allows the NHS ester to covalently attach to the primary amines present in proteins, amine-modified oligonucleotides, and other amine-bearing molecules. Conjugates utilizing XFD660 are noted for their enhanced fluorescence intensity and increased photostability relative to conjugates using other fluorophores with similar spectral characteristics.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 92.645 µL | 463.225 µL | 926.449 µL | 4.632 mL | 9.264 mL |
| 5 mM | 18.529 µL | 92.645 µL | 185.29 µL | 926.449 µL | 1.853 mL |
| 10 mM | 9.264 µL | 46.322 µL | 92.645 µL | 463.225 µL | 926.449 µL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
| Extinction coefficient (cm -1 M -1) | 132000 |
| Excitation (nm) | 663 |
| Emission (nm) | 691 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
| XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
| XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
| XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
| XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
| XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester* | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Show More (11) | ||||||
Images
References
View all 50 references: Citation Explorer
Enhanced fluorescence blinking of AF647 fluorophores in Mowiol via violet and UV light induced recovery for superior localization microscopy.
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Journal: Methods and applications in fluorescence (2024)
Authors: Bharadwaj, Anupam and Kumar, Amalesh and Mitra, Rumela and Jaganathan, Bithiah Grace and Boruah, Bosanta Ranjan
Journal: Methods and applications in fluorescence (2024)
Three species multiplexing of fluorescent dyes and gold nanoclusters recovered with fluorescence lifetime correlation spectroscopy.
Authors: Kayyil Veedu, Malavika and Hajda, Agata and Olesiak-Bańska, Joanna and Wenger, Jérôme
Journal: Biochimica et biophysica acta. General subjects (2024): 130611
Authors: Kayyil Veedu, Malavika and Hajda, Agata and Olesiak-Bańska, Joanna and Wenger, Jérôme
Journal: Biochimica et biophysica acta. General subjects (2024): 130611
Vanadocene-functionalized mesoporous silica nanoparticles: platforms for the development of theranostic materials against breast cancer.
Authors: Iorhemba, Michael Aondona and Álvarez-Conde, Javier and Díaz-García, Diana and Méndez-Arriaga, José Manuel and García-Almodóvar, Victoria and Ovejero-Paredes, Karina and Idris, Sulaiman Ola and Shallangwa, Gideon Adamu and Abdulkadir, Ibrahim and Prashar, Sanjiv and Filice, Marco and Gómez-Ruiz, Santiago
Journal: Biomedical materials (Bristol, England) (2024)
Authors: Iorhemba, Michael Aondona and Álvarez-Conde, Javier and Díaz-García, Diana and Méndez-Arriaga, José Manuel and García-Almodóvar, Victoria and Ovejero-Paredes, Karina and Idris, Sulaiman Ola and Shallangwa, Gideon Adamu and Abdulkadir, Ibrahim and Prashar, Sanjiv and Filice, Marco and Gómez-Ruiz, Santiago
Journal: Biomedical materials (Bristol, England) (2024)
Amphiphilic Poly(2-oxazoline)s with Glycine-Containing Hydrophobic Blocks Tailored for Panobinostat- and Imatinib-Loaded Micelles.
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
USE OF RECOMBINANT S1 PROTEIN WITH hFc FOR ANALYSIS OF SARS-COV-2 ADSORPTION AND EVALUATION OF DRUGS THAT INHIBIT ENTRY INTO VERO E6 CELLS.
Authors: Couto, Jéssica Carla Martins and Vidal, Taís and Decker, Eduardo Reichert and Santurio, Janio M and de Mello, Carlos Fernando and Pillat, Micheli Mainardi
Journal: Immunology letters (2023)
Authors: Couto, Jéssica Carla Martins and Vidal, Taís and Decker, Eduardo Reichert and Santurio, Janio M and de Mello, Carlos Fernando and Pillat, Micheli Mainardi
Journal: Immunology letters (2023)
Rapid and portable bunyavirus SFTSV RNA testing utilizing catalytic hairpin assembly coupled with lateral flow immunoassay.
Authors: Chen, Lin and Ma, Mengyin and Zou, Mingyuan and Zhao, Liwei and Ou, Mingrong and Geng, Yu and Li, Chuang and Shen, Han and Chen, Yuxin
Journal: Microbiology spectrum (2023): e0214423
Authors: Chen, Lin and Ma, Mengyin and Zou, Mingyuan and Zhao, Liwei and Ou, Mingrong and Geng, Yu and Li, Chuang and Shen, Han and Chen, Yuxin
Journal: Microbiology spectrum (2023): e0214423
Chemogenetic inhibition of TrkB signalling reduces phrenic motor neuron survival and size.
Authors: Fogarty, Matthew J and Dasgupta, Debanjali and Khurram, Obaid U and Sieck, Gary C
Journal: Molecular and cellular neurosciences (2023): 103847
Authors: Fogarty, Matthew J and Dasgupta, Debanjali and Khurram, Obaid U and Sieck, Gary C
Journal: Molecular and cellular neurosciences (2023): 103847
Click-Functionalization of Silanized Carbon Nanotubes: From Inorganic Heterostructures to Biosensing Nanohybrids.
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Evaluation of Slowfade Diamond as a buffer for STORM microscopy.
Authors: Boukhatem, Hadjer and Durel, Beatrice and Raimbault, Manon and Laurent, Audrey and Olivier, Nicolas
Journal: Biomedical optics express (2023): 550-558
Authors: Boukhatem, Hadjer and Durel, Beatrice and Raimbault, Manon and Laurent, Audrey and Olivier, Nicolas
Journal: Biomedical optics express (2023): 550-558