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Cy5DIGE NHS ester

Cy5DIGE NHS ester, an equivalent of Cy5® NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy5DIGE is commonly used together with C2DIGE and/or Cy3DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates. Perfect mobility matching and bright dye fluorescence allow the efficient detection and high resolution of minor proteins on 2D gel electrophoresis. Cy2DIGE, C3DIGE and Cy5DIGE are compatible to all imagers capable of detection of Cy2, Cy3, and Cy5 dyes. The gels labeled with Cy2DIGE, C3DIGE and Cy5DIGE are size- and charge-matched fluorescent dyes for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis (DIGE). Cy2DIGE, C3DIGE and Cy5DIGE detect up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. The size- and charge-matched dyes enable co-migration of labeled samples within the gel. They are bright on proteins, allowing the minimal loss of signal during labeling, separation, and scanning. These dyes have minimal spectral overlap, minimizing the crosstalk that commonly contributes to high background. Their pH-insensitive fluorescence allows DIGEs run at a broad pH range. Cy2®, Cy3® and Cy5® are the trademarks of GE Healthcare.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5DIGE NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM141.313 µL706.564 µL1.413 mL7.066 mL14.131 mL
5 mM28.263 µL141.313 µL282.626 µL1.413 mL2.826 mL
10 mM14.131 µL70.656 µL141.313 µL706.564 µL1.413 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*499520710000.300.11
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*343441190000.250.19
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*534553810000.240.09
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*590618900000.430.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]--890001-0.32
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*5535681500000.080.08
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*6506712390000.000.03
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*6817041840000.000.05
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*6967191920000.000.07
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*7527762400000.000.04
Cy2DIGE NHS ester4925081500000.080.15
Cy3DIGE NHS ester55556915000010.070.073
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*5615721120000.210.12
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*579603913000.450.46
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*518543800000.310.18
QXY7 NHS ester [equivalent to QSY-7 NHS ester]--900001-0.22
Cy3B NHS ester56057112000010.0480.069
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]40142135,0000.230.70
CypHer5E NHS Ester643660---
CypHer7E NHS Ester748769---
XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]6636911320000.000.10
XFD430 NHS ester43254015,000-0.28
Show More (13)

Citations

View all 34 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis
Authors: Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C.
Journal: Mol Med Rep (2018): 5571-5580
Proteomic Analysis of Bovine Pregnancy-specific Serum Proteins by 2D Fluorescence Difference Gel Electrophoresis
Authors: Lee, J. E., Lee, J. Y., Kim, H. R., Shin, H. Y., Lin, T., Jin, D. I.
Journal: Asian-Australas J Anim Sci (2015): 788-95
Comparative proteomic analysis of different Toxoplasma gondii genotypes by two-dimensional fluorescence difference gel electrophoresis combined with mass spectrometry
Authors: Zhou, D. H., Zhao, F. R., Nisbet, A. J., Xu, M. J., Song, H. Q., Lin, R. Q., Huang, S. Y., Zhu, X. Q.
Journal: Electrophoresis (2014): 533-45
Comparative proteomic analysis of Dan'er malts produced from distinct malting processes by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE)
Authors: Li, X., Jin, Z., Gao, F., Lu, J., Cai, G., Dong, J., Yu, J., Yang, M.
Journal: J Agric Food Chem (2014): 9310-6
Page updated on October 28, 2024

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Catalog Number25306
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Physical properties

Molecular weight

707.65

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Cy5DIGE NHS ester, an equivalent of Cy5&reg; NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy5DIGE is commonly used together with C2DIGE and/or Cy3DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates.
Cy5DIGE NHS ester, an equivalent of Cy5&reg; NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy5DIGE is commonly used together with C2DIGE and/or Cy3DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates.
Cy5DIGE NHS ester, an equivalent of Cy5&reg; NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy5DIGE is commonly used together with C2DIGE and/or Cy3DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates.