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AAT Bioquest

What are the different types of centrifugation techniques?

Posted May 9, 2024


Answer

There are 8 main types of centrifugation techniques. 

  1. Analytical Centrifugation - In analytical centrifugation, particles in a sample are separated based on their density and the resulting centrifugal force. This centrifugation method is based on the principles that larger molecules move faster and denser particles settle down quicker in the centrifuge. 
  2. Differential Centrifugation - Differential centrifugation exploits differences in the sedimentation rate of biological particles of varying size and density. In this technique, a sample is put through successive increases in centrifugal force, resulting in the components settling down separately. 
  3. Density Gradient Centrifugation - Density gradient centrifugation separates molecules based on the density as they traverse a density gradient under centrifugal force. It relies on the principle that under centrifugal force, molecules settle down until reaching a medium of matching density. 
  4. Rate-zonal density gradient centrifugation/Moving Zone Centrifugation - This centrifugation technique isolates particles based on size and shape. Although it works differently from density gradient centrifugation, both work on the same principle. 
  5. Equilibrium density gradient centrifugation - A modified and specialized form of the density gradient configuration technique, equilibrium density gradient centrifugation separates particles solely based on density, with particles moving through a density gradient and stopping when the medium density equals the density of the particles. 
  6. Isopycnic Centrifugation - Isopycnic centrifugation separates particles based on their densities. This centrifugal technique is also called equilibrium centrifugation as the separation relies solely on particle density, not size. 
  7. Sucrose gradient centrifugation - Sucrose gradient centrifugation employs sucrose-based density gradients to separate molecules. This technique works on the principle that under centrifugal force, molecules settle down when they reach a medium with a matching density. 
  8. Differential velocity (Moving Boundary) centrifugation - Differential velocity centrifugation uses a series of increasing velocities to separate components in a sample. It is based on the principle that particles of varying sizes and densities settle down a centrifuge tube separately at different velocities. 
Additional resources

Creating arrays by centrifugation

Buffers and Lab Consumables

ReadiUse™ Bio-Gel P-6 spin column