What are the applications for each type of centrifugation technique?

Analytical centrifugation is used to: 

• Determine purity of macromolecules in solution
• Track changes in molecular mass of supramolecular complexes
• Establish relative molecular mass of solutes in their native configuration

Differential centrifugation is used to: 

• Separate cell membranes and cellular organelles
• Low –resolution nucleus isolation
• Purify extracts containing larger-sized impurities

Density gradient centrifugation is used to: 

• Purify large volumes of biomolecules
• Purify viruses for further analysis
• Determine densities of different particles 

Rate-zonal density gradient centrifugation is used to: 

• Isolate viruses based on the unique size and density of their components
• Isolate, purify, and fractionate DNA molecules from bacteria and viruses
• Fractionate RNA on sucrose gradients

Equilibrium Density Gradient Centrifugation is used to: 

• Purify large volumes of biomolecules
• Determine density of different particles

Isopycnic centrifugation is used to: 

• Purify large volumes of biomolecules
• Determine densities of different particles

Sucrose Gradient Centrifugation is used to: 

• Separate macromolecules such as DNA and RNA
• Determine the size and density of macromolecules
• Analyze protein complexes

Differential Velocity (Moving Boundary) Centrifugation is used to: 

• Separate cell membranes and organelles
• Low-resolution nucleus isolation
• Identify and compare particles of varying sizes