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AAT Bioquest

Multicolor Live or Dead™ Viability Assays for Flow Cytometry

Image of CHO cells fixed with formaldehyde
Image of CHO cells fixed with formaldehyde and stained with Live or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence* (Cat# 22601) in a Costar 96-well black wall/clear bottom plate.
Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools to labeling cells for fluorescence microscopic and flow cytometric investigations of cellular functions. AAT Bioquest offers a set of multicolor cell labeling kits to uniformly label dead mammalian cells for long term microscopic examination and flow cytometric analysis of cells.

The kits provide all the essential components with an optimized cell-labeling protocol. The proprietary fluorescent dyes used in the kits become more fluorescent upon reacting with dead cellular components. When excited at different excitations using a set of our Live or Dead™ Fixalbe Dead Cell Staining Kits, the dead cell stain emits intense fluorescence ranging from 400 nm to 800 nm. The fluorescent dyes used in the kits have the spectral properties compatible with all the common filter sets. The kits can be readily used with almost every fluorescence instrument often equipped with 405 nm violet laser, 488 nm blue laser or 633 nm red laser sources.

Jurkat cells were treated and stained with live or dead fixable dead cell staining kit green fluorescence
Jurkat cells were stained with live or dead fixable dead cell staining kit red fluorescence

Jurkat cells were treated and stained with Live or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence* 22601 (left) and Live or Dead™ Fixable Dead Cell Staining Kit *Deep Red Fluorescence* 22604 (right). The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 488 nm/520 nm (FL1) channel (left) and 633 nm/660 nm (FL4) channel (right). Nearly identical results were obtained using unfixed cells.