iFluor™ 594, a New Bright Fluorescent Labeling Dye an Excellent Replacement to Texas Red®, DyLight™ 594 and Alexa Fluor® 594 Dyes

iFluor^® 594 has spectral characteristics similar to those of Texas Red^®, DyLight™ 594 and Alexa Fluor^® 594 with excitation and emission wavelength at ~592/614 nm when conjugated to proteins. iFluor^® 594 dye has superior labeling performance and better stability than Texas Red^®. Our iFluor^® 594 conjugated streptavidin provides high fluorescence intensity and low background. Biomolecules conjugated to iFluor^® 594 exhibit little spectral overlap with green-fluorescent conjugates and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser. The minimal spectral overlap makes iFluor^® 594 an ideal second color in combination with a green color such as GFP, FITC, Alexa Fluor^® 488 or iFluor^® 488. Our in-house research indicated that the iFluor^® 594-RPE conjugates demonstrate better FRET than Alexa Fluor^® 594-RPE.

1. *CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm.
2. **CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).