Phalloidin Conjugates

XFD647, produced by AAT Bioquest, shares the identical chemical structure as Alexa Fluor® 647 (Alexa Fluor® being the trademark of ThermoFisher). The XFD647 phalloidin conjugate is essentially equivalent to Alexa Fluor® 647 phalloidin and exhibits a far-red fluorescent signal. This conjugate specifically binds to F-actins, and when used at nanomolar concentrations, it serves as a convenient tool for labeling, identifying, and quantifying F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. The application of fluorescent phalloidin derivatives, such as XFD647, has proven crucial in the detailed examination of actin networks at high resolution. AAT Bioquest provides a range of fluorescent phalloidin derivatives in different colors to facilitate multicolor imaging applications. They are fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot™ nanocrystals, and other iFluor® conjugates, including secondary antibodies.