XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.03 |
Extinction coefficient (cm -1 M -1) | 239000 |
Excitation (nm) | 650 |
Emission (nm) | 671 |
Quantum yield | 0.331 |
Storage, safety and handling
H-phrase | H301, H311, H331 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R23, R24, R25 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | SDSProtocol |
See also: Actin, Cytoskeleton Structure & Analysis
Correction Factor (260 nm) 0.00 | Correction Factor (280 nm) 0.03 | Extinction coefficient (cm -1 M -1) 239000 | Excitation (nm) 650 | Emission (nm) 671 | Quantum yield 0.331 |
XFD647, produced by AAT Bioquest, shares the identical chemical structure as Alexa Fluor® 647 (Alexa Fluor® being the trademark of ThermoFisher). The XFD647 phalloidin conjugate is essentially equivalent to Alexa Fluor® 647 phalloidin and exhibits a far-red fluorescent signal. This conjugate specifically binds to F-actins, and when used at nanomolar concentrations, it serves as a convenient tool for labeling, identifying, and quantifying F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. The application of fluorescent phalloidin derivatives, such as XFD647, has proven crucial in the detailed examination of actin networks at high resolution. AAT Bioquest provides a range of fluorescent phalloidin derivatives in different colors to facilitate multicolor imaging applications. They are fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot™ nanocrystals, and other iFluor® conjugates, including secondary antibodies.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.03 |
Extinction coefficient (cm -1 M -1) | 239000 |
Excitation (nm) | 650 |
Emission (nm) | 671 |
Quantum yield | 0.331 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | - | 0.19 |
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488* | 499 | 520 | 73000 | 0.921 | 0.11 |
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594* | 590 | 618 | 92000 | 0.661 | 0.56 |
XFD647 Azide | 650 | 671 | 270000 | 0.331 | 0.03 |
XFD647 Alkyne | 650 | 671 | 270000 | 0.331 | 0.03 |
XFD647 amine | 650 | 671 | 270000 | 0.331 | 0.03 |
XFD647 acid | 650 | 671 | 270000 | 0.331 | 0.03 |
References
View all 10 references: Citation Explorer
TGFβ2 Induces the Formation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells Through the Smad and Non-Smad Dependent Pathways.
Authors: Montecchi-Palmer, Michela and Bermudez, Jaclyn Y and Webber, Hannah C and Patel, Gaurang C and Clark, Abbot F and Mao, Weiming
Journal: Investigative ophthalmology & visual science (2017): 1288-1295
Authors: Montecchi-Palmer, Michela and Bermudez, Jaclyn Y and Webber, Hannah C and Patel, Gaurang C and Clark, Abbot F and Mao, Weiming
Journal: Investigative ophthalmology & visual science (2017): 1288-1295
Neuroprotective effects of hypothermia on synaptic actin cytoskeletal changes induced by perinatal asphyxia.
Authors: Muñiz, Javier and Romero, Juan and Holubiec, Mariana and Barreto, George and González, Janneth and Saint-Martin, Madeleine and Blanco, Eduardo and Carlos Cavicchia, Juan and Castilla, Rocío and Capani, Francisco
Journal: Brain research (2014): 81-90
Authors: Muñiz, Javier and Romero, Juan and Holubiec, Mariana and Barreto, George and González, Janneth and Saint-Martin, Madeleine and Blanco, Eduardo and Carlos Cavicchia, Juan and Castilla, Rocío and Capani, Francisco
Journal: Brain research (2014): 81-90
Glycation of extracellular matrix proteins impairs migration of immune cells.
Authors: Haucke, Elisa and Navarrete-Santos, Alexander and Simm, Andreas and Silber, Rolf-Edgar and Hofmann, Britt
Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (2014): 239-45
Authors: Haucke, Elisa and Navarrete-Santos, Alexander and Simm, Andreas and Silber, Rolf-Edgar and Hofmann, Britt
Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (2014): 239-45
Effects of perinatal asphyxia on rat striatal cytoskeleton.
Authors: Saraceno, G E and Ayala, M V and Badorrey, M S and Holubiec, M and Romero, J I and Galeano, P and Barreto, G and Giraldez-Alvárez, L D and Kölliker-Fres, R and Coirini, H and Capani, F
Journal: Synapse (New York, N.Y.) (2012): 9-19
Authors: Saraceno, G E and Ayala, M V and Badorrey, M S and Holubiec, M and Romero, J I and Galeano, P and Barreto, G and Giraldez-Alvárez, L D and Kölliker-Fres, R and Coirini, H and Capani, F
Journal: Synapse (New York, N.Y.) (2012): 9-19
Cross-linked actin networks (CLANs) are present in lamina cribrosa cells.
Authors: Job, Raly and Raja, Vignesh and Grierson, Ian and Currie, Laura and O'Reilly, Steven and Pollock, Natalie and Knight, Emma and Clark, Abbot F
Journal: The British journal of ophthalmology (2010): 1388-92
Authors: Job, Raly and Raja, Vignesh and Grierson, Ian and Currie, Laura and O'Reilly, Steven and Pollock, Natalie and Knight, Emma and Clark, Abbot F
Journal: The British journal of ophthalmology (2010): 1388-92
Incidence of protein on actin bridges between endothelium and smooth muscle in arterioles demonstrates heterogeneous connexin expression and phosphorylation.
Authors: Isakson, Brant E and Best, Angela K and Duling, Brian R
Journal: American journal of physiology. Heart and circulatory physiology (2008): H2898-904
Authors: Isakson, Brant E and Best, Angela K and Duling, Brian R
Journal: American journal of physiology. Heart and circulatory physiology (2008): H2898-904
[Uptake and accumulation of gentamicin by mouse hair cells from the cochlear explants in vitro].
Authors: Liu, Jian-Ping and Dai, Chun-Fu and Wang, Zheng-Min and Chi, Fang-Lu and Tian, Jie and Da, Cui-Di
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2008): 439-42
Authors: Liu, Jian-Ping and Dai, Chun-Fu and Wang, Zheng-Min and Chi, Fang-Lu and Tian, Jie and Da, Cui-Di
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2008): 439-42
Depolymerisation and rearrangement of actin filaments during exocytosis in rat peritoneal mast cells: involvement of ryanodine-sensitive calcium stores.
Authors: Guzmán, R E and Bolaños, P and Delgado, A and Rojas, H and DiPolo, R and Caputo, C and Jaffe, E H
Journal: Pflugers Archiv : European journal of physiology (2007): 131-41
Authors: Guzmán, R E and Bolaños, P and Delgado, A and Rojas, H and DiPolo, R and Caputo, C and Jaffe, E H
Journal: Pflugers Archiv : European journal of physiology (2007): 131-41
[Distribution of gentamicin in inner ear after intratympanic gentamicin injection].
Authors: Liu, Jian-Ping and Dai, Chun-Fu and Wang, Zheng-Min and Chi, Fang-Lu and Tian, Jie and Da, Cui-Di
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2006): 851-6
Authors: Liu, Jian-Ping and Dai, Chun-Fu and Wang, Zheng-Min and Chi, Fang-Lu and Tian, Jie and Da, Cui-Di
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2006): 851-6
The protein encoded by the US3 orthologue of Marek's disease virus is required for efficient de-envelopment of perinuclear virions and involved in actin stress fiber breakdown.
Authors: Schumacher, Daniel and Tischer, B Karsten and Trapp, Sascha and Osterrieder, Nikolaus
Journal: Journal of virology (2005): 3987-97
Authors: Schumacher, Daniel and Tischer, B Karsten and Trapp, Sascha and Osterrieder, Nikolaus
Journal: Journal of virology (2005): 3987-97
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?