Phalloidin Conjugates

equivalent to Alexa Fluor® 647 phalloidin

XFD647, produced by AAT Bioquest, shares the identical chemical structure as Alexa Fluor® 647 (Alexa Fluor® being the trademark of ThermoFisher). The XFD647 phalloidin conjugate is essentially equivalent to Alexa Fluor® 647 phalloidin and exhibits a far-red fluorescent signal. This conjugate specifically binds to F-actins, and when used at nanomolar concentrations, it serves as a convenient tool for labeling, identifying, and quantifying F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. The application of fluorescent phalloidin derivatives, such as XFD647, has proven crucial in the detailed examination of actin networks at high resolution. AAT Bioquest provides a range of fluorescent phalloidin derivatives in different colors to facilitate multicolor imaging applications. They are fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot™ nanocrystals, and other iFluor® conjugates, including secondary antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	70.99 µL	354.95 µL	709.9 µL	3.549 mL	7.099 mL
5 mM	14.198 µL	70.99 µL	141.98 µL	709.9 µL	1.42 mL
10 mM	7.099 µL	35.495 µL	70.99 µL	354.95 µL	709.9 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
XFD350 Phalloidin XFD350 Same Structure to Alexa Fluor™ 350	343	441	19000	-	0.19
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.11
XFD594 Phalloidin XFD594 Same Structure to Alexa Fluor™ 594	590	618	92000	0.66¹	0.56
XFD647 Azide	650	671	270000	0.33¹	0.03
XFD647 Alkyne	650	671	270000	0.33¹	0.03
XFD647 amine	650	671	270000	0.33¹	0.03
XFD647 acid	650	671	270000	0.33¹	0.03

References

View all 10 references: Citation Explorer

TGFβ2 Induces the Formation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells Through the Smad and Non-Smad Dependent Pathways.
Authors: Montecchi-Palmer, Michela and Bermudez, Jaclyn Y and Webber, Hannah C and Patel, Gaurang C and Clark, Abbot F and Mao, Weiming
Journal: Investigative ophthalmology & visual science (2017): 1288-1295

Neuroprotective effects of hypothermia on synaptic actin cytoskeletal changes induced by perinatal asphyxia.
Authors: Muñiz, Javier and Romero, Juan and Holubiec, Mariana and Barreto, George and González, Janneth and Saint-Martin, Madeleine and Blanco, Eduardo and Carlos Cavicchia, Juan and Castilla, Rocío and Capani, Francisco
Journal: Brain research (2014): 81-90

Glycation of extracellular matrix proteins impairs migration of immune cells.
Authors: Haucke, Elisa and Navarrete-Santos, Alexander and Simm, Andreas and Silber, Rolf-Edgar and Hofmann, Britt
Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (2014): 239-45

Effects of perinatal asphyxia on rat striatal cytoskeleton.
Authors: Saraceno, G E and Ayala, M V and Badorrey, M S and Holubiec, M and Romero, J I and Galeano, P and Barreto, G and Giraldez-Alvárez, L D and Kölliker-Fres, R and Coirini, H and Capani, F
Journal: Synapse (New York, N.Y.) (2012): 9-19

Cross-linked actin networks (CLANs) are present in lamina cribrosa cells.
Authors: Job, Raly and Raja, Vignesh and Grierson, Ian and Currie, Laura and O'Reilly, Steven and Pollock, Natalie and Knight, Emma and Clark, Abbot F
Journal: The British journal of ophthalmology (2010): 1388-92

Page updated on October 9, 2024

Ordering information

Price
Conjugate
Unit size
Catalog Number	231002310123102231032311023111231152311623117231192312223125231272312823129231302313123140231502315323158231595301
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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	1408.65
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.00
Correction Factor (280 nm)	0.03
Extinction coefficient (cm ^-1 M ^-1)	239000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33¹

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200