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AAT Bioquest

Wheat Germ Agglutinin (WGA)

XFD594 Same Structure to Alexa Fluor™ 594
XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher). Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. XFD594 conjugate of WGA is equivalent to the Alexa Fluor® 594 conjugate of WGA. It exhibits bright, red fluorescence. XFD594 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues.

Spectrum

References

View all 50 references: Citation Explorer
Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427
Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18
Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)
Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815
Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572
Page updated on October 8, 2024

Ordering information

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2545025452254542549525500255022550325509255122551525525255302553225539255502555925560255612556225563
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Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)

592

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.56

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

590

Emission (nm)

618

Quantum yield

0.661

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

Platform

Fluorescence microscope

ExcitationCy3, TRITC filter set
EmissionCy3, TRITC filter set
Recommended plateBlack wall, clear bottom
Live HeLa cells were stained with Wheat Germ Agglutinin, XFD594 labeled at 5 µg/mL for 30 minutes followed by Hoechst 33342 (Cat No. 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.
Live HeLa cells were stained with Wheat Germ Agglutinin, XFD594 labeled at 5 µg/mL for 30 minutes followed by Hoechst 33342 (Cat No. 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.
Live HeLa cells were stained with Wheat Germ Agglutinin, XFD594 labeled at 5 µg/mL for 30 minutes followed by Hoechst 33342 (Cat No. 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.