Wheat Germ Agglutinin (WGA)

XFD488 Same Structure to Alexa Fluor™ 488

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. It is of the most studied and useful lectins for its biological applications. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. The carbohydrate-binding specificity of WGA is directed against sequences of β-1,4-GlcNAc-linked residues, the chitodextrins. Each monomer contains two identical, non-interacting binding sites, which are complementary to 3 or 4 β-1,4-GlcNAc units. Of the monosaccharides examined, only GlcNAc binds to WGA. ManNAc does not bind, and GalNAc binds only weakly. XFD488 conjugate of WGA is equivalent to the Alexa Fluor® 488 conjugate of WGA. It exhibits bright, green fluorescence. XFD488 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Wheat Germ Agglutinin, XFD594 Labeled XFD594 Same Structure to Alexa Fluor™ 594	590	618	90000	0.66¹	0.43	0.56
Wheat Germ Agglutinin, XFD350 Labeled XFD350 Same Structure to Alexa Fluor® 350	343	441	19000	-	0.25	0.19
Wheat Germ Agglutinin, XFD532 Labeled XFD532 Same Structure to Alexa Fluor® 532	534	553	81000	0.61¹	0.24	0.09
Wheat Germ Agglutinin, XFD555 Labeled XFD555 Same Structure to Alexa Fluor® 555	553	568	150000	0.1¹	0.08	0.08
Wheat Germ Agglutinin, XFD647 Labeled XFD647 Same Structure to Alexa Fluor® 647	650	671	239000	0.33¹	0.00	0.03
Wheat Germ Agglutinin, XFD750 Labeled XFD750 Same Structure to Alexa Fluor® 750	752	776	240000	0.12¹	0.00	0.04

References

View all 50 references: Citation Explorer

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572

Page updated on October 8, 2024

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Catalog Number	2545025452254542549525500255022550325509255122551525525255302553225539255502555925560255612556225563
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Physical properties

Solvent

Water

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501