ReadiCleave™ AML Cleavage Buffer *5X Aqueous Solution*
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Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Example protocol
PREPARATION OF WORKING SOLUTION
To prepare a 1X working solution, add 200 μL of ReadiCleave™ AML Cleavage Buffer into 800 μL of ddH2O, and mix thoroughly.
Note: For optimal results, use this solution within a few hours of preparation.
SAMPLE EXPERIMENTAL PROTOCOL
Remove the coverslip by briefly dipping the slides into PBS. Then, wash the tissue sample with PBS to eliminate any remaining mounting medium.
Add 100 µL of ReadiCleave™ AML Cleavage Buffer working solution to the tissue or cell samples.
Note: Add a sufficient amount of ReadiCleave™ AML Cleavage Buffer working solution to ensure that the samples are fully submerged.
Heat the samples at 60°C for 60 minutes.
Remove the ReadiCleave™ AML Cleavage Buffer working solution and briefly rinse the samples with PBST.
Reprocess the tissue samples beginning with the Antigen Retrieval step in your IHC staining protocol.
Images
![Fluorescence imaging for the detection of EpCAM in lung adenocarcinoma was conducted using ReadiCleave™ iFluor® 488 Styramide (Cat# 45500), ReadiCleave™ iFluor® 546 Styramide (Cat# 45510), and ReadiCleave™ iFluor® 647 Styramide (Cat# 45520). Images were captured both before and after treatment with ReadiCleave™ AML Cleavage Buffer, providing comparative insights into the effectiveness of the cleavage buffer treatment.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Freadicleave-aml-cleavage-buffer-5x-aqueous-solution%2Ffigure-for-readicleave-aml-cleavage-buffer-5x-aqueous-solution_zRwi1.png&w=3840&q=75)
References
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