ReadiCleave™ Cy5-SSL-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ Cy5-SSL-NHS ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the Cy5 fluorophore from the target molecule. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
ReadiCleave™ AML Cy5 NHS ester	651	670	250000¹	0.27¹, 0.4²	0.03
ReadiCleave™ XFD532 SSL-NHS ester	534	553	81000	0.61¹	0.09
ReadiCleave™ FAM SSL-NHS ester	491	516	73000	0.92	0.35

References

View all 50 references: Citation Explorer

Repurposing an atherosclerosis targeting peptide for tumor imaging.
Authors: Kovacs, Luciana and Davis, Ryan A and Ganguly, Tanushree and Chammas, Roger and Sutcliffe, Julie L
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2022): 112469

Small molecule based EGFR targeting of biodegradable nanoparticles containing temozolomide and Cy5 dye for greatly enhanced image-guided glioblastoma therapy.
Authors: Schmitt, Rebecca R and Mahajan, Supriya D and Pliss, Artem and Prasad, Paras N
Journal: Nanomedicine : nanotechnology, biology, and medicine (2022): 102513

Nucleic Acid-Gated Covalent Organic Frameworks for Cancer-Specific Imaging and Drug Release.
Authors: Gao, Peng and Shen, Xiaoying and Liu, Xiaohan and Chen, Yuanyuan and Pan, Wei and Li, Na and Tang, Bo
Journal: Analytical chemistry (2021): 11751-11757

Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.
Authors: Abe, Masahiro and Nishihara, Ryo and Kim, Sung-Bae and Suzuki, Koji
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 103-110

On-resin strategy to label α-conotoxins: Cy5-RgIA, a potent α9α10 nicotinic acetylcholine receptor imaging probe.
Authors: Muttenthaler, Markus and Nevin, Simon T and Inserra, Marco and Lewis, Richard J and Adams, David J and Alewood, Paul
Journal: Australian journal of chemistry (2020): 327-333

Page updated on November 16, 2024

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Catalog Number	7050
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Physical properties

Molecular weight	1018.33
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²